Done. -parsing GTF file: /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.yOtAvc2Emi/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.yOtAvc2Emi/fi_workdir/aih-tih-sc-13d441-R1_A23YTGFLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.yOtAvc2Emi/fi_workdir/aih-tih-sc-13d441-R1_A23YTGFLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-13d441-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index aih-tih-sc-13d441-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam 21:56:00 : INFO : Done. -parsing GTF file: /tmp/nxf.yOtAvc2Emi/fi_workdir/aih-tih-sc-13d441-R1_A23YTGFLT4_1.gtf -parsing /tmp/nxf.yOtAvc2Emi/fi_workdir/aih-tih-sc-13d441-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] [500000] [510000] [520000] [530000] [540000] [550000] [560000] [570000] [580000] [590000] [600000] [610000] [620000] [630000] [640000] [650000] [660000] [670000] [680000] [690000] [700000] [710000] [720000] -done parsing /tmp/nxf.yOtAvc2Emi/fi_workdir/aih-tih-sc-13d441-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 17, 'seq-similar region overlap' => 143, 'num genes matched < 2' => 21, 'excessive soft clipping' => 3093, ' ** passed ** ' => 10362, 'per_id < 96' => 2363, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 2, 'small anchor length' => 63, 'low complexity anchor region' => 6, 'exons hit < 2' => 5, 'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 36 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.yOtAvc2Emi/fi_workdir/aih-tih-sc-13d441-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] -fusion SPANNING read extraction for scaff: AC015712.1--FAM13C [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] -fusion SPANNING read extraction for scaff: CRYZL2P--SEC16B [1000] [2000] [3000] -fusion SPANNING read extraction for scaff: KLRC4--KLRK1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: PCDHGA5--PCDHGC4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] -fusion SPANNING read extraction for scaff: PCDHGA5--PCDHGC5 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PCDHGA5--PCDHGA8 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: PCDHGA5--PCDHGB6 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] -fusion SPANNING read extraction for scaff: PCDHGA5--PCDHGA11 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] -fusion SPANNING read extraction for scaff: PCDHGA5--PCDHGA10 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: PCDHGA5--PCDHGA9 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PCDHGA5--PCDHGA12 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] -fusion SPANNING read extraction for scaff: PCDHGA5--PCDHGC3 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] -fusion SPANNING read extraction for scaff: PCDHGA5--PCDHGB7 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC5 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA8 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGB6 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA11 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA10 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA9 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA12 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC3 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGB7 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] -fusion SPANNING read extraction for scaff: RBBP4--FAM13C [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] -fusion SPANNING read extraction for scaff: STX16--NPEPL1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'seq similar region alignment' => 37033, 'lacks exon overlap' => 4928 }; EM: Starting log likelihood: -3058.769487 EM: Round [1] log likelihood: -3057.312958 EM: Round [2] log likelihood: -3056.851556 EM: Round [3] log likelihood: -3056.442342 EM: Round [4] log likelihood: -3056.077253 EM: Round [5] log likelihood: -3055.750910 EM: Round [6] log likelihood: -3055.458692 EM: Round [7] log likelihood: -3055.196609 EM: Round [8] log likelihood: -3054.961204 EM: Round [9] log likelihood: -3054.749467 EM: Round [10] log likelihood: -3054.558770 EM: Round [11] log likelihood: -3054.386812 EM: Round [12] log likelihood: -3054.231571 EM: Round [13] log likelihood: -3054.091267 EM: Round [14] log likelihood: -3053.964331 EM: Round [15] log likelihood: -3053.849375 EM: Round [16] log likelihood: -3053.745167 EM: Round [17] log likelihood: -3053.650617 EM: Round [18] log likelihood: -3053.564754 EM: Round [19] log likelihood: -3053.486712 EM: Round [20] log likelihood: -3053.415723 EM: Round [21] log likelihood: -3053.351097 EM: Round [22] log likelihood: -3053.292219 EM: Round [23] log likelihood: -3053.238538 EM: Round [24] log likelihood: -3053.189561 EM: Round [25] log likelihood: -3053.144844 EM: Round [26] log likelihood: -3053.103990 EM: Round [27] log likelihood: -3053.066641 EM: Round [28] log likelihood: -3053.032475 EM: Round [29] log likelihood: -3053.001201 EM: Round [30] log likelihood: -3052.972558 EM: Round [31] log likelihood: -3052.946310 EM: Round [32] log likelihood: -3052.922242 EM: Round [33] log likelihood: -3052.900163 EM: Round [34] log likelihood: -3052.879898 EM: Round [35] log likelihood: -3052.861287 EM: Round [36] log likelihood: -3052.844189 EM: Round [37] log likelihood: -3052.828472 EM: Round [38] log likelihood: -3052.814019 EM: Round [39] log likelihood: -3052.800722 EM: Round [40] log likelihood: -3052.788484 EM: Round [41] log likelihood: -3052.777215 EM: Round [42] log likelihood: -3052.766836 EM: Round [43] log likelihood: -3052.757271 EM: Round [44] log likelihood: -3052.748455 EM: Round [45] log likelihood: -3052.740326 EM: Round [46] log likelihood: -3052.732827 EM: Round [47] log likelihood: -3052.725907 EM: Round [48] log likelihood: -3052.719521 EM: Round [49] log likelihood: -3052.713625 EM: Round [50] log likelihood: -3052.708179 EM: Round [51] log likelihood: -3052.703149 EM: Round [52] log likelihood: -3052.698500 EM: Round [53] log likelihood: -3052.694204 EM: Round [54] log likelihood: -3052.690232 EM: Round [55] log likelihood: -3052.686560 EM: Round [56] log likelihood: -3052.683163 EM: Round [57] log likelihood: -3052.680020 EM: Round [58] log likelihood: -3052.677113 EM: Round [59] log likelihood: -3052.674422 EM: Round [60] log likelihood: -3052.671931 EM: Round [61] log likelihood: -3052.669625 EM: Round [62] log likelihood: -3052.667490 EM: Round [63] log likelihood: -3052.665513 EM: Round [64] log likelihood: -3052.663681 EM: Round [65] log likelihood: -3052.661984 EM: Round [66] log likelihood: -3052.660412 EM: Round [67] log likelihood: -3052.658956 EM: Round [68] log likelihood: -3052.657606 EM: Round [69] log likelihood: -3052.656354 EM: Round [70] log likelihood: -3052.655194 EM: Round [71] log likelihood: -3052.654119 EM: Round [72] log likelihood: -3052.653122 EM: Round [73] log likelihood: -3052.652197 EM: Round [74] log likelihood: -3052.651340 EM: Round [75] log likelihood: -3052.650545 EM: Round [76] log likelihood: -3052.649807 EM: Round [77] log likelihood: -3052.649123 EM: Round [78] log likelihood: -3052.648488 EM: Round [79] log likelihood: -3052.647899 EM: Round [80] log likelihood: -3052.647353 EM: Round [81] log likelihood: -3052.646846 EM: Round [82] log likelihood: -3052.646375 EM: Round [83] log likelihood: -3052.645939 EM: Round [84] log likelihood: -3052.645533 EM: Round [85] log likelihood: -3052.645157 EM: Round [86] log likelihood: -3052.644808 EM: Round [87] log likelihood: -3052.644484 EM: Round [88] log likelihood: -3052.644183 EM: Round [89] log likelihood: -3052.643904 EM: Round [90] log likelihood: -3052.643645 EM: Round [91] log likelihood: -3052.643404 EM: Round [92] log likelihood: -3052.643181 EM: Round [93] log likelihood: -3052.642973 EM: Round [94] log likelihood: -3052.642781 EM: Round [95] log likelihood: -3052.642602 EM: Round [96] log likelihood: -3052.642436 EM: Round [97] log likelihood: -3052.642281 EM: Round [98] log likelihood: -3052.642138 EM: Round [99] log likelihood: -3052.642005 EM: Round [100] log likelihood: -3052.641882 EM: Round [101] log likelihood: -3052.641767 EM: Round [102] log likelihood: -3052.641660 EM: Round [103] log likelihood: -3052.641561 EM: Stopping iterations at round 103 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.yOtAvc2Emi/fi_workdir/aih-tih-sc-13d441-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.yOtAvc2Emi/fi_workdir/aih-tih-sc-13d441-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.yOtAvc2Emi/fi_workdir/aih-tih-sc-13d441-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -2832.216430 EM: Round [1] log likelihood: -2832.085570 EM: Round [2] log likelihood: -2832.085559 EM: Stopping iterations at round 2 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 257719664 Warning - not locating file: /tmp/nxf.yOtAvc2Emi/aih-tih-sc-13d441-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000232386.9] WARNING, no entry stored in dbm for [ENSG00000242193.11] WARNING, no entry stored in dbm for [ENSG00000242193.11] ls: cannot access 'IGV_inputs': No such file or directory