[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-13d441-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13444256) splicing reads. 268493 splicing junctions. 190914 known splicing junctions. 77579 novel splicing junctions.
sampling 10% (26888513) splicing reads. 328022 splicing junctions. 203630 known splicing junctions. 124392 novel splicing junctions.
sampling 15% (40332769) splicing reads. 370010 splicing junctions. 210424 known splicing junctions. 159586 novel splicing junctions.
sampling 20% (53777026) splicing reads. 402759 splicing junctions. 214958 known splicing junctions. 187801 novel splicing junctions.
sampling 25% (67221283) splicing reads. 430220 splicing junctions. 218289 known splicing junctions. 211931 novel splicing junctions.
sampling 30% (80665539) splicing reads. 453157 splicing junctions. 220823 known splicing junctions. 232334 novel splicing junctions.
sampling 35% (94109796) splicing reads. 472699 splicing junctions. 222803 known splicing junctions. 249896 novel splicing junctions.
sampling 40% (107554053) splicing reads. 489505 splicing junctions. 224441 known splicing junctions. 265064 novel splicing junctions.
sampling 45% (120998309) splicing reads. 504333 splicing junctions. 225805 known splicing junctions. 278528 novel splicing junctions.
sampling 50% (134442566) splicing reads. 517432 splicing junctions. 226934 known splicing junctions. 290498 novel splicing junctions.
sampling 55% (147886823) splicing reads. 528784 splicing junctions. 227940 known splicing junctions. 300844 novel splicing junctions.
sampling 60% (161331079) splicing reads. 538548 splicing junctions. 228740 known splicing junctions. 309808 novel splicing junctions.
sampling 65% (174775336) splicing reads. 547370 splicing junctions. 229431 known splicing junctions. 317939 novel splicing junctions.
sampling 70% (188219593) splicing reads. 554855 splicing junctions. 230016 known splicing junctions. 324839 novel splicing junctions.
sampling 75% (201663849) splicing reads. 561452 splicing junctions. 230507 known splicing junctions. 330945 novel splicing junctions.
sampling 80% (215108106) splicing reads. 567295 splicing junctions. 230987 known splicing junctions. 336308 novel splicing junctions.
sampling 85% (228552363) splicing reads. 572074 splicing junctions. 231360 known splicing junctions. 340714 novel splicing junctions.
sampling 90% (241996619) splicing reads. 576225 splicing junctions. 231684 known splicing junctions. 344541 novel splicing junctions.
sampling 95% (255440876) splicing reads. 579547 splicing junctions. 231933 known splicing junctions. 347614 novel splicing junctions.
sampling 100% (268885133) splicing reads. 582152 splicing junctions. 232125 known splicing junctions. 350027 novel splicing junctions.