[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3abdf4-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10298920) splicing reads. 238153 splicing junctions. 180008 known splicing junctions. 58145 novel splicing junctions.
sampling 10% (20597840) splicing reads. 281933 splicing junctions. 192545 known splicing junctions. 89388 novel splicing junctions.
sampling 15% (30896760) splicing reads. 310487 splicing junctions. 198995 known splicing junctions. 111492 novel splicing junctions.
sampling 20% (41195680) splicing reads. 331357 splicing junctions. 203121 known splicing junctions. 128236 novel splicing junctions.
sampling 25% (51494600) splicing reads. 347424 splicing junctions. 205949 known splicing junctions. 141475 novel splicing junctions.
sampling 30% (61793520) splicing reads. 360469 splicing junctions. 208147 known splicing junctions. 152322 novel splicing junctions.
sampling 35% (72092441) splicing reads. 370966 splicing junctions. 209759 known splicing junctions. 161207 novel splicing junctions.
sampling 40% (82391361) splicing reads. 379729 splicing junctions. 211114 known splicing junctions. 168615 novel splicing junctions.
sampling 45% (92690281) splicing reads. 387061 splicing junctions. 212171 known splicing junctions. 174890 novel splicing junctions.
sampling 50% (102989201) splicing reads. 393341 splicing junctions. 213045 known splicing junctions. 180296 novel splicing junctions.
sampling 55% (113288121) splicing reads. 398777 splicing junctions. 213798 known splicing junctions. 184979 novel splicing junctions.
sampling 60% (123587041) splicing reads. 403355 splicing junctions. 214429 known splicing junctions. 188926 novel splicing junctions.
sampling 65% (133885961) splicing reads. 407175 splicing junctions. 214932 known splicing junctions. 192243 novel splicing junctions.
sampling 70% (144184882) splicing reads. 410474 splicing junctions. 215350 known splicing junctions. 195124 novel splicing junctions.
sampling 75% (154483802) splicing reads. 413294 splicing junctions. 215725 known splicing junctions. 197569 novel splicing junctions.
sampling 80% (164782722) splicing reads. 415512 splicing junctions. 216024 known splicing junctions. 199488 novel splicing junctions.
sampling 85% (175081642) splicing reads. 417375 splicing junctions. 216259 known splicing junctions. 201116 novel splicing junctions.
sampling 90% (185380562) splicing reads. 418947 splicing junctions. 216465 known splicing junctions. 202482 novel splicing junctions.
sampling 95% (195679482) splicing reads. 420094 splicing junctions. 216617 known splicing junctions. 203477 novel splicing junctions.
sampling 100% (205978403) splicing reads. 420903 splicing junctions. 216729 known splicing junctions. 204174 novel splicing junctions.