[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16293190) splicing reads. 268556 splicing junctions. 191345 known splicing junctions. 77211 novel splicing junctions.
sampling 10% (32586380) splicing reads. 328564 splicing junctions. 204386 known splicing junctions. 124178 novel splicing junctions.
sampling 15% (48879570) splicing reads. 372137 splicing junctions. 211584 known splicing junctions. 160553 novel splicing junctions.
sampling 20% (65172761) splicing reads. 406827 splicing junctions. 216480 known splicing junctions. 190347 novel splicing junctions.
sampling 25% (81465951) splicing reads. 435585 splicing junctions. 220074 known splicing junctions. 215511 novel splicing junctions.
sampling 30% (97759141) splicing reads. 460172 splicing junctions. 222712 known splicing junctions. 237460 novel splicing junctions.
sampling 35% (114052332) splicing reads. 481441 splicing junctions. 224855 known splicing junctions. 256586 novel splicing junctions.
sampling 40% (130345522) splicing reads. 500086 splicing junctions. 226697 known splicing junctions. 273389 novel splicing junctions.
sampling 45% (146638712) splicing reads. 516624 splicing junctions. 228137 known splicing junctions. 288487 novel splicing junctions.
sampling 50% (162931903) splicing reads. 531425 splicing junctions. 229430 known splicing junctions. 301995 novel splicing junctions.
sampling 55% (179225093) splicing reads. 544589 splicing junctions. 230436 known splicing junctions. 314153 novel splicing junctions.
sampling 60% (195518283) splicing reads. 555999 splicing junctions. 231313 known splicing junctions. 324686 novel splicing junctions.
sampling 65% (211811473) splicing reads. 566484 splicing junctions. 232105 known splicing junctions. 334379 novel splicing junctions.
sampling 70% (228104664) splicing reads. 575905 splicing junctions. 232800 known splicing junctions. 343105 novel splicing junctions.
sampling 75% (244397854) splicing reads. 583998 splicing junctions. 233389 known splicing junctions. 350609 novel splicing junctions.
sampling 80% (260691044) splicing reads. 591505 splicing junctions. 233933 known splicing junctions. 357572 novel splicing junctions.
sampling 85% (276984235) splicing reads. 598057 splicing junctions. 234424 known splicing junctions. 363633 novel splicing junctions.
sampling 90% (293277425) splicing reads. 603654 splicing junctions. 234810 known splicing junctions. 368844 novel splicing junctions.
sampling 95% (309570615) splicing reads. 608587 splicing junctions. 235178 known splicing junctions. 373409 novel splicing junctions.
sampling 100% (325863806) splicing reads. 612685 splicing junctions. 235458 known splicing junctions. 377227 novel splicing junctions.