Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bd/f46c446ee3afbc48176b612ea190de/aih-tih-sc-3270ab-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d0/db0390e137cf3062481f869034d2c8/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/95/8cbd2775d4db2ea1d8cc6bac80804b/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/95/8cbd2775d4db2ea1d8cc6bac80804b/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/65/b41a656e5398795a74f124141c742a/aih-tih-sc-3270ab-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3270ab-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16289863) splicing reads. 266204 splicing junctions. 189828 known splicing junctions. 76376 novel splicing junctions.
sampling 10% (32579726) splicing reads. 326563 splicing junctions. 203077 known splicing junctions. 123486 novel splicing junctions.
sampling 15% (48869589) splicing reads. 370359 splicing junctions. 210366 known splicing junctions. 159993 novel splicing junctions.
sampling 20% (65159453) splicing reads. 404826 splicing junctions. 215041 known splicing junctions. 189785 novel splicing junctions.
sampling 25% (81449316) splicing reads. 433473 splicing junctions. 218537 known splicing junctions. 214936 novel splicing junctions.
sampling 30% (97739179) splicing reads. 457674 splicing junctions. 221224 known splicing junctions. 236450 novel splicing junctions.
sampling 35% (114029042) splicing reads. 478714 splicing junctions. 223369 known splicing junctions. 255345 novel splicing junctions.
sampling 40% (130318906) splicing reads. 497215 splicing junctions. 225135 known splicing junctions. 272080 novel splicing junctions.
sampling 45% (146608769) splicing reads. 513339 splicing junctions. 226551 known splicing junctions. 286788 novel splicing junctions.
sampling 50% (162898632) splicing reads. 527513 splicing junctions. 227771 known splicing junctions. 299742 novel splicing junctions.
sampling 55% (179188495) splicing reads. 540056 splicing junctions. 228776 known splicing junctions. 311280 novel splicing junctions.
sampling 60% (195478359) splicing reads. 551106 splicing junctions. 229648 known splicing junctions. 321458 novel splicing junctions.
sampling 65% (211768222) splicing reads. 561050 splicing junctions. 230462 known splicing junctions. 330588 novel splicing junctions.
sampling 70% (228058085) splicing reads. 569795 splicing junctions. 231135 known splicing junctions. 338660 novel splicing junctions.
sampling 75% (244347948) splicing reads. 577514 splicing junctions. 231690 known splicing junctions. 345824 novel splicing junctions.
sampling 80% (260637812) splicing reads. 584358 splicing junctions. 232199 known splicing junctions. 352159 novel splicing junctions.
sampling 85% (276927675) splicing reads. 590003 splicing junctions. 232606 known splicing junctions. 357397 novel splicing junctions.
sampling 90% (293217538) splicing reads. 594778 splicing junctions. 232953 known splicing junctions. 361825 novel splicing junctions.
sampling 95% (309507401) splicing reads. 598855 splicing junctions. 233272 known splicing junctions. 365583 novel splicing junctions.
sampling 100% (325797265) splicing reads. 602113 splicing junctions. 233487 known splicing junctions. 368626 novel splicing junctions.
null device 
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