Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/16/06bc43b7a8e03ba958f3ba94bebbb5/aih-tih-sc-24ecfe-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d0/db0390e137cf3062481f869034d2c8/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4f/949c3bf2e107079f2c06625e0e36b7/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/52/1df1663baebbd37ee71760bb555a15/aih-tih-sc-24ecfe-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4f/949c3bf2e107079f2c06625e0e36b7/.command.run
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-24ecfe-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (1034451) splicing reads. 108084 splicing junctions. 101659 known splicing junctions. 6425 novel splicing junctions.
sampling 10% (2068903) splicing reads. 125759 splicing junctions. 116322 known splicing junctions. 9437 novel splicing junctions.
sampling 15% (3103355) splicing reads. 133993 splicing junctions. 122684 known splicing junctions. 11309 novel splicing junctions.
sampling 20% (4137807) splicing reads. 138968 splicing junctions. 126363 known splicing junctions. 12605 novel splicing junctions.
sampling 25% (5172258) splicing reads. 142243 splicing junctions. 128666 known splicing junctions. 13577 novel splicing junctions.
sampling 30% (6206710) splicing reads. 144525 splicing junctions. 130158 known splicing junctions. 14367 novel splicing junctions.
sampling 35% (7241162) splicing reads. 146314 splicing junctions. 131308 known splicing junctions. 15006 novel splicing junctions.
sampling 40% (8275614) splicing reads. 147689 splicing junctions. 132187 known splicing junctions. 15502 novel splicing junctions.
sampling 45% (9310065) splicing reads. 148826 splicing junctions. 132878 known splicing junctions. 15948 novel splicing junctions.
sampling 50% (10344517) splicing reads. 149765 splicing junctions. 133426 known splicing junctions. 16339 novel splicing junctions.
sampling 55% (11378969) splicing reads. 150493 splicing junctions. 133835 known splicing junctions. 16658 novel splicing junctions.
sampling 60% (12413421) splicing reads. 151144 splicing junctions. 134214 known splicing junctions. 16930 novel splicing junctions.
sampling 65% (13447872) splicing reads. 151646 splicing junctions. 134516 known splicing junctions. 17130 novel splicing junctions.
sampling 70% (14482324) splicing reads. 152076 splicing junctions. 134765 known splicing junctions. 17311 novel splicing junctions.
sampling 75% (15516776) splicing reads. 152393 splicing junctions. 134944 known splicing junctions. 17449 novel splicing junctions.
sampling 80% (16551228) splicing reads. 152660 splicing junctions. 135098 known splicing junctions. 17562 novel splicing junctions.
sampling 85% (17585679) splicing reads. 152848 splicing junctions. 135206 known splicing junctions. 17642 novel splicing junctions.
sampling 90% (18620131) splicing reads. 153008 splicing junctions. 135296 known splicing junctions. 17712 novel splicing junctions.
sampling 95% (19654583) splicing reads. 153092 splicing junctions. 135345 known splicing junctions. 17747 novel splicing junctions.
sampling 100% (20689035) splicing reads. 153142 splicing junctions. 135374 known splicing junctions. 17768 novel splicing junctions.
null device 
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