Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d0/db0390e137cf3062481f869034d2c8/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/18/c3f49e03cc6f9c43b348995ac66e0e/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/18/c3f49e03cc6f9c43b348995ac66e0e/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/30/3cf4336b6d6237aae68dc4a0d3f4c1/aih-tih-sc-242a7d-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c2/68a0e4fe9b712d598a431d27357208/aih-tih-sc-242a7d-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-242a7d-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12484852) splicing reads. 221022 splicing junctions. 173781 known splicing junctions. 47241 novel splicing junctions.
sampling 10% (24969704) splicing reads. 248546 splicing junctions. 182466 known splicing junctions. 66080 novel splicing junctions.
sampling 15% (37454557) splicing reads. 263305 splicing junctions. 186224 known splicing junctions. 77081 novel splicing junctions.
sampling 20% (49939409) splicing reads. 272700 splicing junctions. 188456 known splicing junctions. 84244 novel splicing junctions.
sampling 25% (62424261) splicing reads. 279489 splicing junctions. 189894 known splicing junctions. 89595 novel splicing junctions.
sampling 30% (74909114) splicing reads. 284469 splicing junctions. 190918 known splicing junctions. 93551 novel splicing junctions.
sampling 35% (87393966) splicing reads. 288263 splicing junctions. 191676 known splicing junctions. 96587 novel splicing junctions.
sampling 40% (99878818) splicing reads. 291267 splicing junctions. 192231 known splicing junctions. 99036 novel splicing junctions.
sampling 45% (112363671) splicing reads. 293670 splicing junctions. 192702 known splicing junctions. 100968 novel splicing junctions.
sampling 50% (124848523) splicing reads. 295763 splicing junctions. 193081 known splicing junctions. 102682 novel splicing junctions.
sampling 55% (137333375) splicing reads. 297543 splicing junctions. 193389 known splicing junctions. 104154 novel splicing junctions.
sampling 60% (149818228) splicing reads. 298986 splicing junctions. 193621 known splicing junctions. 105365 novel splicing junctions.
sampling 65% (162303080) splicing reads. 300235 splicing junctions. 193833 known splicing junctions. 106402 novel splicing junctions.
sampling 70% (174787932) splicing reads. 301262 splicing junctions. 193995 known splicing junctions. 107267 novel splicing junctions.
sampling 75% (187272785) splicing reads. 302109 splicing junctions. 194140 known splicing junctions. 107969 novel splicing junctions.
sampling 80% (199757637) splicing reads. 302825 splicing junctions. 194251 known splicing junctions. 108574 novel splicing junctions.
sampling 85% (212242489) splicing reads. 303384 splicing junctions. 194350 known splicing junctions. 109034 novel splicing junctions.
sampling 90% (224727342) splicing reads. 303804 splicing junctions. 194412 known splicing junctions. 109392 novel splicing junctions.
sampling 95% (237212194) splicing reads. 304136 splicing junctions. 194464 known splicing junctions. 109672 novel splicing junctions.
sampling 100% (249697047) splicing reads. 304348 splicing junctions. 194490 known splicing junctions. 109858 novel splicing junctions.
null device 
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