[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13136763) splicing reads. 254079 splicing junctions. 180540 known splicing junctions. 73539 novel splicing junctions.
sampling 10% (26273526) splicing reads. 311237 splicing junctions. 193111 known splicing junctions. 118126 novel splicing junctions.
sampling 15% (39410289) splicing reads. 351932 splicing junctions. 199750 known splicing junctions. 152182 novel splicing junctions.
sampling 20% (52547052) splicing reads. 383650 splicing junctions. 204431 known splicing junctions. 179219 novel splicing junctions.
sampling 25% (65683816) splicing reads. 408827 splicing junctions. 207575 known splicing junctions. 201252 novel splicing junctions.
sampling 30% (78820579) splicing reads. 429670 splicing junctions. 210018 known splicing junctions. 219652 novel splicing junctions.
sampling 35% (91957342) splicing reads. 447176 splicing junctions. 211896 known splicing junctions. 235280 novel splicing junctions.
sampling 40% (105094105) splicing reads. 462151 splicing junctions. 213496 known splicing junctions. 248655 novel splicing junctions.
sampling 45% (118230868) splicing reads. 475209 splicing junctions. 214808 known splicing junctions. 260401 novel splicing junctions.
sampling 50% (131367632) splicing reads. 486476 splicing junctions. 215844 known splicing junctions. 270632 novel splicing junctions.
sampling 55% (144504395) splicing reads. 496202 splicing junctions. 216696 known splicing junctions. 279506 novel splicing junctions.
sampling 60% (157641158) splicing reads. 504600 splicing junctions. 217449 known splicing junctions. 287151 novel splicing junctions.
sampling 65% (170777921) splicing reads. 511852 splicing junctions. 218071 known splicing junctions. 293781 novel splicing junctions.
sampling 70% (183914684) splicing reads. 518174 splicing junctions. 218563 known splicing junctions. 299611 novel splicing junctions.
sampling 75% (197051448) splicing reads. 523419 splicing junctions. 218998 known splicing junctions. 304421 novel splicing junctions.
sampling 80% (210188211) splicing reads. 527840 splicing junctions. 219339 known splicing junctions. 308501 novel splicing junctions.
sampling 85% (223324974) splicing reads. 531472 splicing junctions. 219674 known splicing junctions. 311798 novel splicing junctions.
sampling 90% (236461737) splicing reads. 534453 splicing junctions. 219931 known splicing junctions. 314522 novel splicing junctions.
sampling 95% (249598500) splicing reads. 536675 splicing junctions. 220108 known splicing junctions. 316567 novel splicing junctions.
sampling 100% (262735264) splicing reads. 538179 splicing junctions. 220250 known splicing junctions. 317929 novel splicing junctions.