[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0754d2-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (899669) splicing reads. 105992 splicing junctions. 96231 known splicing junctions. 9761 novel splicing junctions.
sampling 10% (1799339) splicing reads. 124496 splicing junctions. 109995 known splicing junctions. 14501 novel splicing junctions.
sampling 15% (2699008) splicing reads. 133543 splicing junctions. 116220 known splicing junctions. 17323 novel splicing junctions.
sampling 20% (3598678) splicing reads. 138855 splicing junctions. 119613 known splicing junctions. 19242 novel splicing junctions.
sampling 25% (4498347) splicing reads. 142416 splicing junctions. 121846 known splicing junctions. 20570 novel splicing junctions.
sampling 30% (5398017) splicing reads. 145041 splicing junctions. 123423 known splicing junctions. 21618 novel splicing junctions.
sampling 35% (6297686) splicing reads. 147012 splicing junctions. 124582 known splicing junctions. 22430 novel splicing junctions.
sampling 40% (7197356) splicing reads. 148458 splicing junctions. 125387 known splicing junctions. 23071 novel splicing junctions.
sampling 45% (8097025) splicing reads. 149646 splicing junctions. 126036 known splicing junctions. 23610 novel splicing junctions.
sampling 50% (8996695) splicing reads. 150597 splicing junctions. 126537 known splicing junctions. 24060 novel splicing junctions.
sampling 55% (9896364) splicing reads. 151347 splicing junctions. 126953 known splicing junctions. 24394 novel splicing junctions.
sampling 60% (10796034) splicing reads. 151976 splicing junctions. 127300 known splicing junctions. 24676 novel splicing junctions.
sampling 65% (11695703) splicing reads. 152479 splicing junctions. 127576 known splicing junctions. 24903 novel splicing junctions.
sampling 70% (12595373) splicing reads. 152957 splicing junctions. 127819 known splicing junctions. 25138 novel splicing junctions.
sampling 75% (13495042) splicing reads. 153302 splicing junctions. 127991 known splicing junctions. 25311 novel splicing junctions.
sampling 80% (14394712) splicing reads. 153579 splicing junctions. 128113 known splicing junctions. 25466 novel splicing junctions.
sampling 85% (15294381) splicing reads. 153783 splicing junctions. 128206 known splicing junctions. 25577 novel splicing junctions.
sampling 90% (16194051) splicing reads. 153946 splicing junctions. 128284 known splicing junctions. 25662 novel splicing junctions.
sampling 95% (17093720) splicing reads. 154034 splicing junctions. 128320 known splicing junctions. 25714 novel splicing junctions.
sampling 100% (17993390) splicing reads. 154087 splicing junctions. 128347 known splicing junctions. 25740 novel splicing junctions.