Done. -parsing GTF file: /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.dIpS1I9D8d/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.dIpS1I9D8d/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.dIpS1I9D8d/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam 21:37:50 : INFO : Done. -parsing GTF file: /tmp/nxf.dIpS1I9D8d/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.gtf -parsing /tmp/nxf.dIpS1I9D8d/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] -done parsing /tmp/nxf.dIpS1I9D8d/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 11, 'per_id < 96' => 364, ' ** passed ** ' => 1042, 'excessive soft clipping' => 913, 'seq-similar region overlap' => 37, 'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 30, 'low complexity anchor region' => 1 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.dIpS1I9D8d/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] -fusion SPANNING read extraction for scaff: PRCP--RAB30 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] -fusion SPANNING read extraction for scaff: TLK2--AL669831.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'seq similar region alignment' => 10336, 'lacks exon overlap' => 644 }; EM: Starting log likelihood: -506.204039 EM: Round [1] log likelihood: -506.188481 EM: Round [2] log likelihood: -506.173288 EM: Round [3] log likelihood: -506.158451 EM: Round [4] log likelihood: -506.143958 EM: Round [5] log likelihood: -506.129798 EM: Round [6] log likelihood: -506.115962 EM: Round [7] log likelihood: -506.102441 EM: Round [8] log likelihood: -506.089224 EM: Round [9] log likelihood: -506.076303 EM: Round [10] log likelihood: -506.063671 EM: Round [11] log likelihood: -506.051317 EM: Round [12] log likelihood: -506.039236 EM: Round [13] log likelihood: -506.027418 EM: Round [14] log likelihood: -506.015858 EM: Round [15] log likelihood: -506.004547 EM: Round [16] log likelihood: -505.993479 EM: Round [17] log likelihood: -505.982648 EM: Round [18] log likelihood: -505.972047 EM: Round [19] log likelihood: -505.961670 EM: Round [20] log likelihood: -505.951512 EM: Round [21] log likelihood: -505.941566 EM: Round [22] log likelihood: -505.931827 EM: Round [23] log likelihood: -505.922290 EM: Round [24] log likelihood: -505.912950 EM: Round [25] log likelihood: -505.903802 EM: Round [26] log likelihood: -505.894841 EM: Round [27] log likelihood: -505.886062 EM: Round [28] log likelihood: -505.877461 EM: Round [29] log likelihood: -505.869034 EM: Round [30] log likelihood: -505.860776 EM: Round [31] log likelihood: -505.852684 EM: Round [32] log likelihood: -505.844752 EM: Round [33] log likelihood: -505.836979 EM: Round [34] log likelihood: -505.829358 EM: Round [35] log likelihood: -505.821889 EM: Round [36] log likelihood: -505.814565 EM: Round [37] log likelihood: -505.807385 EM: Round [38] log likelihood: -505.800345 EM: Round [39] log likelihood: -505.793442 EM: Round [40] log likelihood: -505.786672 EM: Round [41] log likelihood: -505.780033 EM: Round [42] log likelihood: -505.773522 EM: Round [43] log likelihood: -505.767135 EM: Round [44] log likelihood: -505.760871 EM: Round [45] log likelihood: -505.754725 EM: Round [46] log likelihood: -505.748697 EM: Round [47] log likelihood: -505.742782 EM: Round [48] log likelihood: -505.736980 EM: Round [49] log likelihood: -505.731286 EM: Round [50] log likelihood: -505.725699 EM: Round [51] log likelihood: -505.720217 EM: Round [52] log likelihood: -505.714838 EM: Round [53] log likelihood: -505.709558 EM: Round [54] log likelihood: -505.704377 EM: Round [55] log likelihood: -505.699291 EM: Round [56] log likelihood: -505.694300 EM: Round [57] log likelihood: -505.689400 EM: Round [58] log likelihood: -505.684590 EM: Round [59] log likelihood: -505.679869 EM: Round [60] log likelihood: -505.675234 EM: Round [61] log likelihood: -505.670684 EM: Round [62] log likelihood: -505.666217 EM: Round [63] log likelihood: -505.661830 EM: Round [64] log likelihood: -505.657524 EM: Round [65] log likelihood: -505.653295 EM: Round [66] log likelihood: -505.649143 EM: Round [67] log likelihood: -505.645065 EM: Round [68] log likelihood: -505.641061 EM: Round [69] log likelihood: -505.637129 EM: Round [70] log likelihood: -505.633267 EM: Round [71] log likelihood: -505.629474 EM: Round [72] log likelihood: -505.625749 EM: Round [73] log likelihood: -505.622090 EM: Round [74] log likelihood: -505.618497 EM: Round [75] log likelihood: -505.614967 EM: Round [76] log likelihood: -505.611499 EM: Round [77] log likelihood: -505.608093 EM: Round [78] log likelihood: -505.604748 EM: Round [79] log likelihood: -505.601461 EM: Round [80] log likelihood: -505.598232 EM: Round [81] log likelihood: -505.595059 EM: Round [82] log likelihood: -505.591943 EM: Round [83] log likelihood: -505.588881 EM: Round [84] log likelihood: -505.585872 EM: Round [85] log likelihood: -505.582917 EM: Round [86] log likelihood: -505.580012 EM: Round [87] log likelihood: -505.577158 EM: Round [88] log likelihood: -505.574354 EM: Round [89] log likelihood: -505.571599 EM: Round [90] log likelihood: -505.568891 EM: Round [91] log likelihood: -505.566230 EM: Round [92] log likelihood: -505.563616 EM: Round [93] log likelihood: -505.561046 EM: Round [94] log likelihood: -505.558521 EM: Round [95] log likelihood: -505.556039 EM: Round [96] log likelihood: -505.553600 EM: Round [97] log likelihood: -505.551203 EM: Round [98] log likelihood: -505.548847 EM: Round [99] log likelihood: -505.546532 EM: Round [100] log likelihood: -505.544256 EM: Round [101] log likelihood: -505.542019 EM: Round [102] log likelihood: -505.539820 EM: Round [103] log likelihood: -505.537659 EM: Round [104] log likelihood: -505.535535 EM: Round [105] log likelihood: -505.533447 EM: Round [106] log likelihood: -505.531394 EM: Round [107] log likelihood: -505.529376 EM: Round [108] log likelihood: -505.527393 EM: Round [109] log likelihood: -505.525443 EM: Round [110] log likelihood: -505.523526 EM: Round [111] log likelihood: -505.521642 EM: Round [112] log likelihood: -505.519790 EM: Round [113] log likelihood: -505.517969 EM: Round [114] log likelihood: -505.516178 EM: Round [115] log likelihood: -505.514418 EM: Round [116] log likelihood: -505.512688 EM: Round [117] log likelihood: -505.510987 EM: Round [118] log likelihood: -505.509314 EM: Round [119] log likelihood: -505.507670 EM: Round [120] log likelihood: -505.506053 EM: Round [121] log likelihood: -505.504463 EM: Round [122] log likelihood: -505.502900 EM: Round [123] log likelihood: -505.501363 EM: Round [124] log likelihood: -505.499852 EM: Round [125] log likelihood: -505.498366 EM: Round [126] log likelihood: -505.496905 EM: Round [127] log likelihood: -505.495468 EM: Round [128] log likelihood: -505.494056 EM: Round [129] log likelihood: -505.492667 EM: Round [130] log likelihood: -505.491301 EM: Round [131] log likelihood: -505.489958 EM: Round [132] log likelihood: -505.488637 EM: Round [133] log likelihood: -505.487338 EM: Round [134] log likelihood: -505.486061 EM: Round [135] log likelihood: -505.484805 EM: Round [136] log likelihood: -505.483570 EM: Round [137] log likelihood: -505.482356 EM: Round [138] log likelihood: -505.481161 EM: Round [139] log likelihood: -505.479987 EM: Round [140] log likelihood: -505.478832 EM: Round [141] log likelihood: -505.477696 EM: Round [142] log likelihood: -505.476579 EM: Round [143] log likelihood: -505.475480 EM: Round [144] log likelihood: -505.474399 EM: Round [145] log likelihood: -505.473337 EM: Round [146] log likelihood: -505.472292 EM: Round [147] log likelihood: -505.471264 EM: Round [148] log likelihood: -505.470253 EM: Round [149] log likelihood: -505.469259 EM: Round [150] log likelihood: -505.468281 EM: Round [151] log likelihood: -505.467319 EM: Round [152] log likelihood: -505.466373 EM: Round [153] log likelihood: -505.465443 EM: Round [154] log likelihood: -505.464528 EM: Round [155] log likelihood: -505.463628 EM: Round [156] log likelihood: -505.462743 EM: Round [157] log likelihood: -505.461872 EM: Round [158] log likelihood: -505.461016 EM: Round [159] log likelihood: -505.460174 EM: Round [160] log likelihood: -505.459345 EM: Round [161] log likelihood: -505.458530 EM: Round [162] log likelihood: -505.457729 EM: Round [163] log likelihood: -505.456941 EM: Round [164] log likelihood: -505.456165 EM: Round [165] log likelihood: -505.455402 EM: Round [166] log likelihood: -505.454652 EM: Round [167] log likelihood: -505.453914 EM: Round [168] log likelihood: -505.453188 EM: Round [169] log likelihood: -505.452474 EM: Round [170] log likelihood: -505.451772 EM: Round [171] log likelihood: -505.451081 EM: Round [172] log likelihood: -505.450402 EM: Round [173] log likelihood: -505.449733 EM: Round [174] log likelihood: -505.449075 EM: Round [175] log likelihood: -505.448429 EM: Round [176] log likelihood: -505.447792 EM: Round [177] log likelihood: -505.447166 EM: Round [178] log likelihood: -505.446551 EM: Round [179] log likelihood: -505.445945 EM: Round [180] log likelihood: -505.445349 EM: Round [181] log likelihood: -505.444763 EM: Round [182] log likelihood: -505.444187 EM: Round [183] log likelihood: -505.443620 EM: Round [184] log likelihood: -505.443062 EM: Round [185] log likelihood: -505.442513 EM: Round [186] log likelihood: -505.441973 EM: Round [187] log likelihood: -505.441442 EM: Round [188] log likelihood: -505.440919 EM: Round [189] log likelihood: -505.440405 EM: Round [190] log likelihood: -505.439900 EM: Round [191] log likelihood: -505.439402 EM: Round [192] log likelihood: -505.438913 EM: Round [193] log likelihood: -505.438431 EM: Round [194] log likelihood: -505.437958 EM: Round [195] log likelihood: -505.437492 EM: Round [196] log likelihood: -505.437033 EM: Round [197] log likelihood: -505.436583 EM: Round [198] log likelihood: -505.436139 EM: Round [199] log likelihood: -505.435703 EM: Round [200] log likelihood: -505.435273 EM: Round [201] log likelihood: -505.434851 EM: Round [202] log likelihood: -505.434435 EM: Round [203] log likelihood: -505.434027 EM: Round [204] log likelihood: -505.433624 EM: Round [205] log likelihood: -505.433229 EM: Round [206] log likelihood: -505.432839 EM: Round [207] log likelihood: -505.432457 EM: Round [208] log likelihood: -505.432080 EM: Round [209] log likelihood: -505.431709 EM: Round [210] log likelihood: -505.431344 EM: Round [211] log likelihood: -505.430986 EM: Round [212] log likelihood: -505.430633 EM: Round [213] log likelihood: -505.430286 EM: Round [214] log likelihood: -505.429944 EM: Round [215] log likelihood: -505.429608 EM: Round [216] log likelihood: -505.429277 EM: Round [217] log likelihood: -505.428952 EM: Round [218] log likelihood: -505.428632 EM: Round [219] log likelihood: -505.428317 EM: Round [220] log likelihood: -505.428007 EM: Round [221] log likelihood: -505.427702 EM: Round [222] log likelihood: -505.427402 EM: Round [223] log likelihood: -505.427107 EM: Round [224] log likelihood: -505.426817 EM: Round [225] log likelihood: -505.426531 EM: Round [226] log likelihood: -505.426250 EM: Round [227] log likelihood: -505.425974 EM: Round [228] log likelihood: -505.425702 EM: Round [229] log likelihood: -505.425434 EM: Round [230] log likelihood: -505.425171 EM: Round [231] log likelihood: -505.424912 EM: Round [232] log likelihood: -505.424657 EM: Round [233] log likelihood: -505.424406 EM: Round [234] log likelihood: -505.424160 EM: Round [235] log likelihood: -505.423917 EM: Round [236] log likelihood: -505.423678 EM: Round [237] log likelihood: -505.423443 EM: Round [238] log likelihood: -505.423212 EM: Round [239] log likelihood: -505.422984 EM: Round [240] log likelihood: -505.422761 EM: Round [241] log likelihood: -505.422541 EM: Round [242] log likelihood: -505.422324 EM: Round [243] log likelihood: -505.422111 EM: Round [244] log likelihood: -505.421901 EM: Round [245] log likelihood: -505.421695 EM: Round [246] log likelihood: -505.421492 EM: Round [247] log likelihood: -505.421292 EM: Round [248] log likelihood: -505.421095 EM: Round [249] log likelihood: -505.420902 EM: Round [250] log likelihood: -505.420712 EM: Round [251] log likelihood: -505.420524 EM: Round [252] log likelihood: -505.420340 EM: Round [253] log likelihood: -505.420159 EM: Round [254] log likelihood: -505.419981 EM: Round [255] log likelihood: -505.419805 EM: Round [256] log likelihood: -505.419633 EM: Round [257] log likelihood: -505.419463 EM: Round [258] log likelihood: -505.419296 EM: Round [259] log likelihood: -505.419131 EM: Round [260] log likelihood: -505.418970 EM: Round [261] log likelihood: -505.418810 EM: Round [262] log likelihood: -505.418654 EM: Round [263] log likelihood: -505.418500 EM: Round [264] log likelihood: -505.418348 EM: Round [265] log likelihood: -505.418199 EM: Round [266] log likelihood: -505.418052 EM: Round [267] log likelihood: -505.417908 EM: Round [268] log likelihood: -505.417765 EM: Round [269] log likelihood: -505.417626 EM: Round [270] log likelihood: -505.417488 EM: Round [271] log likelihood: -505.417353 EM: Round [272] log likelihood: -505.417220 EM: Round [273] log likelihood: -505.417088 EM: Round [274] log likelihood: -505.416960 EM: Round [275] log likelihood: -505.416833 EM: Round [276] log likelihood: -505.416708 EM: Round [277] log likelihood: -505.416585 EM: Round [278] log likelihood: -505.416464 EM: Round [279] log likelihood: -505.416345 EM: Round [280] log likelihood: -505.416228 EM: Round [281] log likelihood: -505.416113 EM: Round [282] log likelihood: -505.416000 EM: Round [283] log likelihood: -505.415888 EM: Round [284] log likelihood: -505.415779 EM: Round [285] log likelihood: -505.415671 EM: Round [286] log likelihood: -505.415564 EM: Round [287] log likelihood: -505.415460 EM: Round [288] log likelihood: -505.415357 EM: Round [289] log likelihood: -505.415256 EM: Round [290] log likelihood: -505.415157 EM: Stopping iterations at round 290 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.dIpS1I9D8d/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.dIpS1I9D8d/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.dIpS1I9D8d/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -505.409045 EM: Round [1] log likelihood: -505.409045 EM: Stopping iterations at round 1 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 216439276 Warning - not locating file: /tmp/nxf.dIpS1I9D8d/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000228327.3] WARNING, no entry stored in dbm for [ENSG00000228327.3] ls: cannot access 'IGV_inputs': No such file or directory