[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-150e73-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14265007) splicing reads. 260847 splicing junctions. 186280 known splicing junctions. 74567 novel splicing junctions.
sampling 10% (28530015) splicing reads. 316362 splicing junctions. 198879 known splicing junctions. 117483 novel splicing junctions.
sampling 15% (42795022) splicing reads. 354800 splicing junctions. 205727 known splicing junctions. 149073 novel splicing junctions.
sampling 20% (57060030) splicing reads. 384321 splicing junctions. 210098 known splicing junctions. 174223 novel splicing junctions.
sampling 25% (71325037) splicing reads. 408350 splicing junctions. 213202 known splicing junctions. 195148 novel splicing junctions.
sampling 30% (85590045) splicing reads. 428244 splicing junctions. 215605 known splicing junctions. 212639 novel splicing junctions.
sampling 35% (99855052) splicing reads. 444994 splicing junctions. 217544 known splicing junctions. 227450 novel splicing junctions.
sampling 40% (114120060) splicing reads. 459264 splicing junctions. 219108 known splicing junctions. 240156 novel splicing junctions.
sampling 45% (128385067) splicing reads. 471605 splicing junctions. 220374 known splicing junctions. 251231 novel splicing junctions.
sampling 50% (142650075) splicing reads. 482070 splicing junctions. 221407 known splicing junctions. 260663 novel splicing junctions.
sampling 55% (156915083) splicing reads. 491230 splicing junctions. 222273 known splicing junctions. 268957 novel splicing junctions.
sampling 60% (171180090) splicing reads. 499151 splicing junctions. 223040 known splicing junctions. 276111 novel splicing junctions.
sampling 65% (185445098) splicing reads. 506204 splicing junctions. 223717 known splicing junctions. 282487 novel splicing junctions.
sampling 70% (199710105) splicing reads. 512168 splicing junctions. 224254 known splicing junctions. 287914 novel splicing junctions.
sampling 75% (213975113) splicing reads. 517455 splicing junctions. 224777 known splicing junctions. 292678 novel splicing junctions.
sampling 80% (228240120) splicing reads. 521953 splicing junctions. 225165 known splicing junctions. 296788 novel splicing junctions.
sampling 85% (242505128) splicing reads. 525689 splicing junctions. 225481 known splicing junctions. 300208 novel splicing junctions.
sampling 90% (256770135) splicing reads. 528904 splicing junctions. 225766 known splicing junctions. 303138 novel splicing junctions.
sampling 95% (271035143) splicing reads. 531427 splicing junctions. 225986 known splicing junctions. 305441 novel splicing junctions.
sampling 100% (285300151) splicing reads. 533326 splicing junctions. 226132 known splicing junctions. 307194 novel splicing junctions.