[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-184b05-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14827826) splicing reads. 267115 splicing junctions. 187727 known splicing junctions. 79388 novel splicing junctions.
sampling 10% (29655652) splicing reads. 324847 splicing junctions. 200277 known splicing junctions. 124570 novel splicing junctions.
sampling 15% (44483478) splicing reads. 364874 splicing junctions. 206985 known splicing junctions. 157889 novel splicing junctions.
sampling 20% (59311305) splicing reads. 395706 splicing junctions. 211356 known splicing junctions. 184350 novel splicing junctions.
sampling 25% (74139131) splicing reads. 420566 splicing junctions. 214516 known splicing junctions. 206050 novel splicing junctions.
sampling 30% (88966957) splicing reads. 441557 splicing junctions. 216921 known splicing junctions. 224636 novel splicing junctions.
sampling 35% (103794784) splicing reads. 459141 splicing junctions. 218782 known splicing junctions. 240359 novel splicing junctions.
sampling 40% (118622610) splicing reads. 474209 splicing junctions. 220318 known splicing junctions. 253891 novel splicing junctions.
sampling 45% (133450436) splicing reads. 487172 splicing junctions. 221548 known splicing junctions. 265624 novel splicing junctions.
sampling 50% (148278263) splicing reads. 498478 splicing junctions. 222675 known splicing junctions. 275803 novel splicing junctions.
sampling 55% (163106089) splicing reads. 508189 splicing junctions. 223506 known splicing junctions. 284683 novel splicing junctions.
sampling 60% (177933915) splicing reads. 516565 splicing junctions. 224237 known splicing junctions. 292328 novel splicing junctions.
sampling 65% (192761741) splicing reads. 523842 splicing junctions. 224859 known splicing junctions. 298983 novel splicing junctions.
sampling 70% (207589568) splicing reads. 530338 splicing junctions. 225389 known splicing junctions. 304949 novel splicing junctions.
sampling 75% (222417394) splicing reads. 535851 splicing junctions. 225852 known splicing junctions. 309999 novel splicing junctions.
sampling 80% (237245220) splicing reads. 540690 splicing junctions. 226242 known splicing junctions. 314448 novel splicing junctions.
sampling 85% (252073047) splicing reads. 544602 splicing junctions. 226547 known splicing junctions. 318055 novel splicing junctions.
sampling 90% (266900873) splicing reads. 547951 splicing junctions. 226820 known splicing junctions. 321131 novel splicing junctions.
sampling 95% (281728699) splicing reads. 550555 splicing junctions. 227051 known splicing junctions. 323504 novel splicing junctions.
sampling 100% (296556526) splicing reads. 552608 splicing junctions. 227207 known splicing junctions. 325401 novel splicing junctions.