Done. -parsing GTF file: /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.nPMYJDuozx/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.nPMYJDuozx/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.nPMYJDuozx/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam 22:38:55 : INFO : Done. -parsing GTF file: /tmp/nxf.nPMYJDuozx/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.gtf -parsing /tmp/nxf.nPMYJDuozx/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] -done parsing /tmp/nxf.nPMYJDuozx/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'excessive soft clipping' => 733, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 10, 'seq-similar region overlap' => 19, ' ** passed ** ' => 671, 'per_id < 96' => 347, 'num genes matched < 2' => 2, 'exons hit < 2' => 2 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.nPMYJDuozx/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1 [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 691, 'seq similar region alignment' => 3815 }; EM: Starting log likelihood: -701.559623 EM: Round [1] log likelihood: -701.489914 EM: Round [2] log likelihood: -701.471009 EM: Round [3] log likelihood: -701.453260 EM: Round [4] log likelihood: -701.436573 EM: Round [5] log likelihood: -701.420867 EM: Round [6] log likelihood: -701.406072 EM: Round [7] log likelihood: -701.392122 EM: Round [8] log likelihood: -701.378958 EM: Round [9] log likelihood: -701.366527 EM: Round [10] log likelihood: -701.354779 EM: Round [11] log likelihood: -701.343669 EM: Round [12] log likelihood: -701.333157 EM: Round [13] log likelihood: -701.323204 EM: Round [14] log likelihood: -701.313776 EM: Round [15] log likelihood: -701.304840 EM: Round [16] log likelihood: -701.296367 EM: Round [17] log likelihood: -701.288328 EM: Round [18] log likelihood: -701.280699 EM: Round [19] log likelihood: -701.273456 EM: Round [20] log likelihood: -701.266577 EM: Round [21] log likelihood: -701.260041 EM: Round [22] log likelihood: -701.253828 EM: Round [23] log likelihood: -701.247922 EM: Round [24] log likelihood: -701.242305 EM: Round [25] log likelihood: -701.236961 EM: Round [26] log likelihood: -701.231877 EM: Round [27] log likelihood: -701.227037 EM: Round [28] log likelihood: -701.222430 EM: Round [29] log likelihood: -701.218043 EM: Round [30] log likelihood: -701.213864 EM: Round [31] log likelihood: -701.209884 EM: Round [32] log likelihood: -701.206091 EM: Round [33] log likelihood: -701.202476 EM: Round [34] log likelihood: -701.199031 EM: Round [35] log likelihood: -701.195747 EM: Round [36] log likelihood: -701.192616 EM: Round [37] log likelihood: -701.189630 EM: Round [38] log likelihood: -701.186782 EM: Round [39] log likelihood: -701.184065 EM: Round [40] log likelihood: -701.181474 EM: Round [41] log likelihood: -701.179001 EM: Round [42] log likelihood: -701.176642 EM: Round [43] log likelihood: -701.174390 EM: Round [44] log likelihood: -701.172241 EM: Round [45] log likelihood: -701.170190 EM: Round [46] log likelihood: -701.168232 EM: Round [47] log likelihood: -701.166362 EM: Round [48] log likelihood: -701.164577 EM: Round [49] log likelihood: -701.162873 EM: Round [50] log likelihood: -701.161246 EM: Round [51] log likelihood: -701.159692 EM: Round [52] log likelihood: -701.158207 EM: Round [53] log likelihood: -701.156789 EM: Round [54] log likelihood: -701.155435 EM: Round [55] log likelihood: -701.154141 EM: Round [56] log likelihood: -701.152905 EM: Round [57] log likelihood: -701.151725 EM: Round [58] log likelihood: -701.150597 EM: Round [59] log likelihood: -701.149519 EM: Round [60] log likelihood: -701.148489 EM: Round [61] log likelihood: -701.147505 EM: Round [62] log likelihood: -701.146564 EM: Round [63] log likelihood: -701.145666 EM: Round [64] log likelihood: -701.144807 EM: Round [65] log likelihood: -701.143986 EM: Round [66] log likelihood: -701.143201 EM: Round [67] log likelihood: -701.142452 EM: Round [68] log likelihood: -701.141735 EM: Round [69] log likelihood: -701.141050 EM: Round [70] log likelihood: -701.140395 EM: Round [71] log likelihood: -701.139769 EM: Round [72] log likelihood: -701.139171 EM: Round [73] log likelihood: -701.138599 EM: Round [74] log likelihood: -701.138052 EM: Round [75] log likelihood: -701.137529 EM: Round [76] log likelihood: -701.137029 EM: Round [77] log likelihood: -701.136551 EM: Round [78] log likelihood: -701.136094 EM: Round [79] log likelihood: -701.135657 EM: Round [80] log likelihood: -701.135240 EM: Round [81] log likelihood: -701.134841 EM: Round [82] log likelihood: -701.134459 EM: Round [83] log likelihood: -701.134094 EM: Round [84] log likelihood: -701.133745 EM: Round [85] log likelihood: -701.133411 EM: Round [86] log likelihood: -701.133092 EM: Round [87] log likelihood: -701.132786 EM: Round [88] log likelihood: -701.132495 EM: Round [89] log likelihood: -701.132216 EM: Round [90] log likelihood: -701.131949 EM: Round [91] log likelihood: -701.131693 EM: Round [92] log likelihood: -701.131449 EM: Round [93] log likelihood: -701.131216 EM: Round [94] log likelihood: -701.130993 EM: Round [95] log likelihood: -701.130779 EM: Round [96] log likelihood: -701.130575 EM: Round [97] log likelihood: -701.130380 EM: Round [98] log likelihood: -701.130194 EM: Round [99] log likelihood: -701.130015 EM: Round [100] log likelihood: -701.129844 EM: Round [101] log likelihood: -701.129681 EM: Round [102] log likelihood: -701.129525 EM: Round [103] log likelihood: -701.129376 EM: Round [104] log likelihood: -701.129233 EM: Round [105] log likelihood: -701.129096 EM: Round [106] log likelihood: -701.128965 EM: Round [107] log likelihood: -701.128841 EM: Round [108] log likelihood: -701.128721 EM: Round [109] log likelihood: -701.128607 EM: Round [110] log likelihood: -701.128497 EM: Round [111] log likelihood: -701.128393 EM: Round [112] log likelihood: -701.128293 EM: Stopping iterations at round 112 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.nPMYJDuozx/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.nPMYJDuozx/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.nPMYJDuozx/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -656.365350 EM: Round [1] log likelihood: -656.315789 EM: Round [2] log likelihood: -656.315782 EM: Stopping iterations at round 2 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 296375716 Warning - not locating file: /tmp/nxf.nPMYJDuozx/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000280136.2] ls: cannot access 'IGV_inputs': No such file or directory