+ mkdir aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai 107516it [00:00, 1074835.57it/s] 220730it [00:00, 1108506.29it/s] 333528it [00:00, 1117371.38it/s] 447630it [00:00, 1126683.02it/s] 561205it [00:00, 1129925.64it/s] 675204it [00:00, 1133331.19it/s] 788538it [00:00, 1131599.56it/s] 903774it [00:00, 1138180.37it/s] 1018124it [00:00, 1139825.48it/s] 1132322it [00:01, 1140472.99it/s] 1246370it [00:01, 1138047.42it/s] 1360177it [00:01, 1136471.46it/s] 1473826it [00:01, 1135328.64it/s] 1587360it [00:01, 1130734.62it/s] 1703570it [00:01, 1140053.32it/s] 1818060it [00:01, 1141365.86it/s] 1932658it [00:01, 1142739.87it/s] 2046936it [00:01, 1139767.61it/s] 2162466it [00:01, 1144302.39it/s] 2276901it [00:02, 1138620.53it/s] 2390772it [00:02, 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1148828.24it/s] 5838783it [00:05, 1146724.76it/s] 5953767it [00:05, 1147593.87it/s] 6068528it [00:05, 1146894.58it/s] 6183219it [00:05, 1145619.01it/s] 6298067it [00:05, 1146456.45it/s] 6412714it [00:05, 1141827.87it/s] 6526901it [00:05, 1139962.68it/s] 6640901it [00:05, 1139560.84it/s] 6755913it [00:05, 1142701.84it/s] 6870186it [00:06, 1139774.41it/s] 6984925it [00:06, 1142037.40it/s] 7101373it [00:06, 1148740.49it/s] 7216485it [00:06, 1149438.76it/s] 7331432it [00:06, 1146870.67it/s] 7446122it [00:06, 1146062.80it/s] 7562813it [00:06, 1152204.34it/s] 7678367it [00:06, 1153186.50it/s] 7793688it [00:06, 1151761.22it/s] 7909632it [00:06, 1154044.91it/s] 8025038it [00:07, 1149982.14it/s] 8140041it [00:07, 1147142.63it/s] 8254759it [00:07, 1141727.57it/s] 8369182it [00:07, 1142454.53it/s] 8485315it [00:07, 1148072.61it/s] 8600129it [00:07, 1146848.40it/s] 8714818it [00:07, 1143370.27it/s] 8829627it [00:07, 1144681.98it/s] 8944300it [00:07, 1145276.92it/s] 9058831it [00:07, 1144346.21it/s] 9173268it [00:08, 1141223.96it/s] 9287394it [00:08, 1138194.01it/s] 9401217it [00:08, 1138161.31it/s] 9515570it [00:08, 1139748.76it/s] 9630063it [00:08, 1141226.07it/s] 9744188it [00:08, 1138999.04it/s] 9859027it [00:08, 1141791.88it/s] 9973360it [00:08, 1142232.46it/s] 10087585it [00:08, 1141363.96it/s] 10201723it [00:08, 1138948.89it/s] 10315620it [00:09, 1138719.92it/s] 10430457it [00:09, 1141538.97it/s] 10545663it [00:09, 1144670.91it/s] 10564286it [00:09, 1142743.16it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1/aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1.md -output aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1/aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1/aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1/aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1_full_basic_stats.tsv