[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12528339) splicing reads. 252369 splicing junctions. 185511 known splicing junctions. 66858 novel splicing junctions.
sampling 10% (25056679) splicing reads. 306040 splicing junctions. 198260 known splicing junctions. 107780 novel splicing junctions.
sampling 15% (37585018) splicing reads. 344017 splicing junctions. 205099 known splicing junctions. 138918 novel splicing junctions.
sampling 20% (50113358) splicing reads. 373622 splicing junctions. 209553 known splicing junctions. 164069 novel splicing junctions.
sampling 25% (62641698) splicing reads. 397573 splicing junctions. 212771 known splicing junctions. 184802 novel splicing junctions.
sampling 30% (75170037) splicing reads. 417893 splicing junctions. 215258 known splicing junctions. 202635 novel splicing junctions.
sampling 35% (87698377) splicing reads. 435067 splicing junctions. 217270 known splicing junctions. 217797 novel splicing junctions.
sampling 40% (100226717) splicing reads. 449926 splicing junctions. 218853 known splicing junctions. 231073 novel splicing junctions.
sampling 45% (112755056) splicing reads. 462730 splicing junctions. 220142 known splicing junctions. 242588 novel splicing junctions.
sampling 50% (125283396) splicing reads. 473730 splicing junctions. 221222 known splicing junctions. 252508 novel splicing junctions.
sampling 55% (137811736) splicing reads. 483315 splicing junctions. 222136 known splicing junctions. 261179 novel splicing junctions.
sampling 60% (150340075) splicing reads. 491880 splicing junctions. 222944 known splicing junctions. 268936 novel splicing junctions.
sampling 65% (162868415) splicing reads. 499296 splicing junctions. 223635 known splicing junctions. 275661 novel splicing junctions.
sampling 70% (175396755) splicing reads. 505623 splicing junctions. 224225 known splicing junctions. 281398 novel splicing junctions.
sampling 75% (187925094) splicing reads. 511139 splicing junctions. 224724 known splicing junctions. 286415 novel splicing junctions.
sampling 80% (200453434) splicing reads. 515788 splicing junctions. 225146 known splicing junctions. 290642 novel splicing junctions.
sampling 85% (212981774) splicing reads. 519730 splicing junctions. 225478 known splicing junctions. 294252 novel splicing junctions.
sampling 90% (225510113) splicing reads. 522934 splicing junctions. 225768 known splicing junctions. 297166 novel splicing junctions.
sampling 95% (238038453) splicing reads. 525485 splicing junctions. 225983 known splicing junctions. 299502 novel splicing junctions.
sampling 100% (250566793) splicing reads. 527362 splicing junctions. 226144 known splicing junctions. 301218 novel splicing junctions.