[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-31d31f-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (6938883) splicing reads. 204185 splicing junctions. 166944 known splicing junctions. 37241 novel splicing junctions.
sampling 10% (13877766) splicing reads. 230186 splicing junctions. 176521 known splicing junctions. 53665 novel splicing junctions.
sampling 15% (20816649) splicing reads. 244428 splicing junctions. 180815 known splicing junctions. 63613 novel splicing junctions.
sampling 20% (27755532) splicing reads. 253773 splicing junctions. 183317 known splicing junctions. 70456 novel splicing junctions.
sampling 25% (34694415) splicing reads. 260419 splicing junctions. 185023 known splicing junctions. 75396 novel splicing junctions.
sampling 30% (41633298) splicing reads. 265465 splicing junctions. 186229 known splicing junctions. 79236 novel splicing junctions.
sampling 35% (48572182) splicing reads. 269279 splicing junctions. 187053 known splicing junctions. 82226 novel splicing junctions.
sampling 40% (55511065) splicing reads. 272404 splicing junctions. 187744 known splicing junctions. 84660 novel splicing junctions.
sampling 45% (62449948) splicing reads. 274974 splicing junctions. 188315 known splicing junctions. 86659 novel splicing junctions.
sampling 50% (69388831) splicing reads. 277014 splicing junctions. 188740 known splicing junctions. 88274 novel splicing junctions.
sampling 55% (76327714) splicing reads. 278727 splicing junctions. 189076 known splicing junctions. 89651 novel splicing junctions.
sampling 60% (83266597) splicing reads. 280245 splicing junctions. 189368 known splicing junctions. 90877 novel splicing junctions.
sampling 65% (90205480) splicing reads. 281488 splicing junctions. 189622 known splicing junctions. 91866 novel splicing junctions.
sampling 70% (97144364) splicing reads. 282516 splicing junctions. 189822 known splicing junctions. 92694 novel splicing junctions.
sampling 75% (104083247) splicing reads. 283359 splicing junctions. 189983 known splicing junctions. 93376 novel splicing junctions.
sampling 80% (111022130) splicing reads. 284044 splicing junctions. 190108 known splicing junctions. 93936 novel splicing junctions.
sampling 85% (117961013) splicing reads. 284611 splicing junctions. 190191 known splicing junctions. 94420 novel splicing junctions.
sampling 90% (124899896) splicing reads. 285105 splicing junctions. 190267 known splicing junctions. 94838 novel splicing junctions.
sampling 95% (131838779) splicing reads. 285410 splicing junctions. 190332 known splicing junctions. 95078 novel splicing junctions.
sampling 100% (138777663) splicing reads. 285628 splicing junctions. 190361 known splicing junctions. 95267 novel splicing junctions.