+ mkdir aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai 151442it [00:00, 1514255.37it/s] 317022it [00:00, 1597451.36it/s] 485743it [00:00, 1638387.74it/s] 655721it [00:00, 1662580.44it/s] 825323it [00:00, 1674597.26it/s] 995207it [00:00, 1682799.06it/s] 1164906it [00:00, 1687399.26it/s] 1336202it [00:00, 1695498.10it/s] 1508990it [00:00, 1705586.87it/s] 1679549it [00:01, 1702396.98it/s] 1849791it [00:01, 1690794.41it/s] 2020725it [00:01, 1696370.37it/s] 2192214it [00:01, 1701926.95it/s] 2362982it [00:01, 1703585.66it/s] 2533350it [00:01, 1697068.38it/s] 2706634it [00:01, 1707762.97it/s] 2883317it [00:01, 1725437.02it/s] 3055875it [00:01, 1720833.34it/s] 3227970it [00:01, 1713970.69it/s] 3399380it [00:02, 1691575.01it/s] 3572683it [00:02, 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1713689.43it/s] 8694307it [00:05, 1712738.48it/s] 8865583it [00:05, 1711547.74it/s] 9036739it [00:05, 1699673.72it/s] 9207191it [00:05, 1701074.06it/s] 9377313it [00:05, 1697897.95it/s] 9547618it [00:05, 1699401.46it/s] 9719789it [00:05, 1706037.66it/s] 9891713it [00:05, 1709957.90it/s] 10064577it [00:05, 1715514.59it/s] 10236134it [00:06, 1713353.76it/s] 10410635it [00:06, 1722800.26it/s] 10582920it [00:06, 1718876.10it/s] 10754813it [00:06, 1716084.94it/s] 10926426it [00:06, 1714146.43it/s] 11097844it [00:06, 1709296.03it/s] 11268778it [00:06, 1699342.02it/s] 11441898it [00:06, 1708799.38it/s] 11613440it [00:06, 1710632.82it/s] 11784515it [00:06, 1710609.82it/s] 11955584it [00:07, 1709376.82it/s] 12126528it [00:07, 1706321.39it/s] 12297165it [00:07, 1703799.02it/s] 12467549it [00:07, 1701545.17it/s] 12639009it [00:07, 1705414.94it/s] 12810453it [00:07, 1708078.13it/s] 12983497it [00:07, 1714748.84it/s] 13154975it [00:07, 1710136.33it/s] 13325993it [00:07, 1708716.40it/s] 13496868it [00:07, 1704663.28it/s] 13508536it [00:07, 1700660.67it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1/aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md -output aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1/aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1/aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1/aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1_full_basic_stats.tsv