Done.
-parsing GTF file: /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf
* Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.O1J2x8c046/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.O1J2x8c046/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.O1J2x8c046/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c'
* Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam
* Running CMD: samtools index aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam
21:55:36 : INFO : Done.
-parsing GTF file: /tmp/nxf.O1J2x8c046/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf
-parsing /tmp/nxf.O1J2x8c046/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam
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[1170000] -done parsing /tmp/nxf.O1J2x8c046/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info.
junction read elimination tally: $VAR1 = {
'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 6,
'seq-similar region overlap' => 98,
'small anchor length' => 1,
'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 3,
'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 53,
'num genes matched < 2' => 12,
'per_id < 96' => 4571,
' ** passed ** ' => 3077,
'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 21,
'excessive soft clipping' => 7635,
'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 20,
'low complexity anchor region' => 2
};
-writing fusion junction support info.
-outputting the spanning read info: /tmp/nxf.O1J2x8c046/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info.
- counting read alignments among fusion contigs.
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[58000] -fusion SPANNING read extraction for scaff: FSIP1--AC013652.1
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[73000] -fusion SPANNING read extraction for scaff: WWP2--MPHOSPH6
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[203000] -fusion SPANNING read extraction for scaff: ZFHX3--CNTNAP4
-filtered reads reasons: $VAR1 = {
'seq similar region alignment' => 19027,
'lacks exon overlap' => 46966
};
EM: Starting log likelihood: -2786.763736
EM: Round [1] log likelihood: -2786.453495
EM: Round [2] log likelihood: -2786.168151
EM: Round [3] log likelihood: -2785.905189
EM: Round [4] log likelihood: -2785.662421
EM: Round [5] log likelihood: -2785.437939
EM: Round [6] log likelihood: -2785.230063
EM: Round [7] log likelihood: -2785.037310
EM: Round [8] log likelihood: -2784.858363
EM: Round [9] log likelihood: -2784.692052
EM: Round [10] log likelihood: -2784.537328
EM: Round [11] log likelihood: -2784.393250
EM: Round [12] log likelihood: -2784.258972
EM: Round [13] log likelihood: -2784.133728
EM: Round [14] log likelihood: -2784.016826
EM: Round [15] log likelihood: -2783.907638
EM: Round [16] log likelihood: -2783.805591
EM: Round [17] log likelihood: -2783.710164
EM: Round [18] log likelihood: -2783.620879
EM: Round [19] log likelihood: -2783.537301
EM: Round [20] log likelihood: -2783.459028
EM: Round [21] log likelihood: -2783.385693
EM: Round [22] log likelihood: -2783.316956
EM: Round [23] log likelihood: -2783.252506
EM: Round [24] log likelihood: -2783.192054
EM: Round [25] log likelihood: -2783.135334
EM: Round [26] log likelihood: -2783.082100
EM: Round [27] log likelihood: -2783.032123
EM: Round [28] log likelihood: -2782.985191
EM: Round [29] log likelihood: -2782.941109
EM: Round [30] log likelihood: -2782.899694
EM: Round [31] log likelihood: -2782.860776
EM: Round [32] log likelihood: -2782.824197
EM: Round [33] log likelihood: -2782.789810
EM: Round [34] log likelihood: -2782.757478
EM: Round [35] log likelihood: -2782.727073
EM: Round [36] log likelihood: -2782.698476
EM: Round [37] log likelihood: -2782.671575
EM: Round [38] log likelihood: -2782.646267
EM: Round [39] log likelihood: -2782.622453
EM: Round [40] log likelihood: -2782.600044
EM: Round [41] log likelihood: -2782.578953
EM: Round [42] log likelihood: -2782.559102
EM: Round [43] log likelihood: -2782.540415
EM: Round [44] log likelihood: -2782.522822
EM: Round [45] log likelihood: -2782.506258
EM: Round [46] log likelihood: -2782.490662
EM: Round [47] log likelihood: -2782.475975
EM: Round [48] log likelihood: -2782.462144
EM: Round [49] log likelihood: -2782.449118
EM: Round [50] log likelihood: -2782.436849
EM: Round [51] log likelihood: -2782.425293
EM: Round [52] log likelihood: -2782.414407
EM: Round [53] log likelihood: -2782.404153
EM: Round [54] log likelihood: -2782.394492
EM: Round [55] log likelihood: -2782.385391
EM: Round [56] log likelihood: -2782.376816
EM: Round [57] log likelihood: -2782.368736
EM: Round [58] log likelihood: -2782.361124
EM: Round [59] log likelihood: -2782.353950
EM: Round [60] log likelihood: -2782.347191
EM: Round [61] log likelihood: -2782.340821
EM: Round [62] log likelihood: -2782.334818
EM: Round [63] log likelihood: -2782.329161
EM: Round [64] log likelihood: -2782.323830
EM: Round [65] log likelihood: -2782.318806
EM: Round [66] log likelihood: -2782.314070
EM: Round [67] log likelihood: -2782.309607
EM: Round [68] log likelihood: -2782.305401
EM: Round [69] log likelihood: -2782.301436
EM: Round [70] log likelihood: -2782.297699
EM: Round [71] log likelihood: -2782.294177
EM: Round [72] log likelihood: -2782.290856
EM: Round [73] log likelihood: -2782.287727
EM: Round [74] log likelihood: -2782.284777
EM: Round [75] log likelihood: -2782.281996
EM: Round [76] log likelihood: -2782.279374
EM: Round [77] log likelihood: -2782.276903
EM: Round [78] log likelihood: -2782.274574
EM: Round [79] log likelihood: -2782.272378
EM: Round [80] log likelihood: -2782.270308
EM: Round [81] log likelihood: -2782.268357
EM: Round [82] log likelihood: -2782.266517
EM: Round [83] log likelihood: -2782.264783
EM: Round [84] log likelihood: -2782.263148
EM: Round [85] log likelihood: -2782.261607
EM: Round [86] log likelihood: -2782.260154
EM: Round [87] log likelihood: -2782.258784
EM: Round [88] log likelihood: -2782.257493
EM: Round [89] log likelihood: -2782.256275
EM: Round [90] log likelihood: -2782.255128
EM: Round [91] log likelihood: -2782.254046
EM: Round [92] log likelihood: -2782.253025
EM: Round [93] log likelihood: -2782.252064
EM: Round [94] log likelihood: -2782.251157
EM: Round [95] log likelihood: -2782.250302
EM: Round [96] log likelihood: -2782.249496
EM: Round [97] log likelihood: -2782.248736
EM: Round [98] log likelihood: -2782.248020
EM: Round [99] log likelihood: -2782.247344
EM: Round [100] log likelihood: -2782.246708
EM: Round [101] log likelihood: -2782.246107
EM: Round [102] log likelihood: -2782.245541
EM: Round [103] log likelihood: -2782.245007
EM: Round [104] log likelihood: -2782.244504
EM: Round [105] log likelihood: -2782.244030
EM: Round [106] log likelihood: -2782.243583
EM: Round [107] log likelihood: -2782.243161
EM: Round [108] log likelihood: -2782.242763
EM: Round [109] log likelihood: -2782.242389
EM: Round [110] log likelihood: -2782.242035
EM: Round [111] log likelihood: -2782.241702
EM: Round [112] log likelihood: -2782.241388
EM: Round [113] log likelihood: -2782.241092
EM: Round [114] log likelihood: -2782.240812
EM: Round [115] log likelihood: -2782.240549
EM: Round [116] log likelihood: -2782.240301
EM: Round [117] log likelihood: -2782.240067
EM: Round [118] log likelihood: -2782.239846
EM: Round [119] log likelihood: -2782.239638
EM: Round [120] log likelihood: -2782.239442
EM: Round [121] log likelihood: -2782.239257
EM: Round [122] log likelihood: -2782.239082
EM: Round [123] log likelihood: -2782.238918
EM: Round [124] log likelihood: -2782.238763
EM: Round [125] log likelihood: -2782.238617
EM: Round [126] log likelihood: -2782.238479
EM: Round [127] log likelihood: -2782.238349
EM: Round [128] log likelihood: -2782.238226
EM: Round [129] log likelihood: -2782.238111
EM: Round [130] log likelihood: -2782.238002
EM: Round [131] log likelihood: -2782.237899
EM: Round [132] log likelihood: -2782.237803
EM: Stopping iterations at round 132 due to insufficient improvement in likelihood.
[bam_sort_core] merging from 0 files and 8 in-memory blocks...
-extracting read coordinates from /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.read_coords
CMD: touch /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.O1J2x8c046/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.O1J2x8c046/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
CMD: cp /tmp/nxf.O1J2x8c046/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter
-done, see /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass
EM: Starting log likelihood: -2611.979692
EM: Round [1] log likelihood: -2611.979692
EM: Stopping iterations at round 1 due to insufficient improvement in likelihood.
-total frags in /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 241112810
Warning - not locating file: /tmp/nxf.O1J2x8c046/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed
WARNING, no entry stored in dbm for [ENSG00000280136.2]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000259345.7]
WARNING, no entry stored in dbm for [ENSG00000259345.7]
WARNING, no entry stored in dbm for [ENSG00000259345.7]
ls: cannot access 'IGV_inputs': No such file or directory