Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44380657575(97.9705%) Q30 bases: 42719256727(94.303%) Read2 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44326221070(97.8504%) Q30 bases: 42316771041(93.4145%) Read1 after filtering: total reads: 296219478 total bases: 36946022195 Q20 bases: 36764155965(99.5078%) Q30 bases: 35951666684(97.3086%) Read2 after filtering: total reads: 296219478 total bases: 36988021550 Q20 bases: 36706007858(99.2376%) Q30 bases: 35704438607(96.5297%) Filtering result: reads passed filter: 592438956 reads failed due to low quality: 7342632 reads failed due to too many N: 24442 reads failed due to too short: 193970 reads with adapter trimmed: 372331622 bases trimmed due to adapters: 15646540703 Duplication rate: 43.9835% Insert size peak (evaluated by paired-end reads): 111 JSON report: aih-tih-sc-eecf9f-R1_A23YTGFLT4_1.fastp.json HTML report: aih-tih-sc-eecf9f-R1_A23YTGFLT4_1.fastp.html fastp --in1 aih-tih-sc-eecf9f-R1_A23YTGFLT4_1_1.fastq.gz --in2 aih-tih-sc-eecf9f-R1_A23YTGFLT4_1_2.fastq.gz --out1 aih-tih-sc-eecf9f-R1_A23YTGFLT4_1_1.fastp.fastq.gz --out2 aih-tih-sc-eecf9f-R1_A23YTGFLT4_1_2.fastp.fastq.gz --json aih-tih-sc-eecf9f-R1_A23YTGFLT4_1.fastp.json --html aih-tih-sc-eecf9f-R1_A23YTGFLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 935 seconds