Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f5/2ae5b378c12aadd2e2caa877425391/aih-tih-sc-3377ac-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d0/db0390e137cf3062481f869034d2c8/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/99/5958319892cd58fcfa53df0c3e058a/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/24/da9dd178c5c216644dfa0d72c2ff44/aih-tih-sc-3377ac-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/99/5958319892cd58fcfa53df0c3e058a/.command.run
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16422855) splicing reads. 242368 splicing junctions. 180973 known splicing junctions. 61395 novel splicing junctions.
sampling 10% (32845711) splicing reads. 289063 splicing junctions. 192943 known splicing junctions. 96120 novel splicing junctions.
sampling 15% (49268566) splicing reads. 320613 splicing junctions. 199274 known splicing junctions. 121339 novel splicing junctions.
sampling 20% (65691422) splicing reads. 344427 splicing junctions. 203511 known splicing junctions. 140916 novel splicing junctions.
sampling 25% (82114277) splicing reads. 362795 splicing junctions. 206368 known splicing junctions. 156427 novel splicing junctions.
sampling 30% (98537133) splicing reads. 377818 splicing junctions. 208554 known splicing junctions. 169264 novel splicing junctions.
sampling 35% (114959988) splicing reads. 390321 splicing junctions. 210357 known splicing junctions. 179964 novel splicing junctions.
sampling 40% (131382844) splicing reads. 400912 splicing junctions. 211800 known splicing junctions. 189112 novel splicing junctions.
sampling 45% (147805699) splicing reads. 409905 splicing junctions. 212983 known splicing junctions. 196922 novel splicing junctions.
sampling 50% (164228555) splicing reads. 417512 splicing junctions. 213855 known splicing junctions. 203657 novel splicing junctions.
sampling 55% (180651410) splicing reads. 424106 splicing junctions. 214614 known splicing junctions. 209492 novel splicing junctions.
sampling 60% (197074266) splicing reads. 429729 splicing junctions. 215204 known splicing junctions. 214525 novel splicing junctions.
sampling 65% (213497121) splicing reads. 434579 splicing junctions. 215769 known splicing junctions. 218810 novel splicing junctions.
sampling 70% (229919977) splicing reads. 438590 splicing junctions. 216222 known splicing junctions. 222368 novel splicing junctions.
sampling 75% (246342832) splicing reads. 442012 splicing junctions. 216593 known splicing junctions. 225419 novel splicing junctions.
sampling 80% (262765688) splicing reads. 444922 splicing junctions. 216916 known splicing junctions. 228006 novel splicing junctions.
sampling 85% (279188543) splicing reads. 447267 splicing junctions. 217185 known splicing junctions. 230082 novel splicing junctions.
sampling 90% (295611399) splicing reads. 449151 splicing junctions. 217392 known splicing junctions. 231759 novel splicing junctions.
sampling 95% (312034254) splicing reads. 450532 splicing junctions. 217563 known splicing junctions. 232969 novel splicing junctions.
sampling 100% (328457110) splicing reads. 451511 splicing junctions. 217666 known splicing junctions. 233845 novel splicing junctions.
null device 
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