[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16641234) splicing reads. 270568 splicing junctions. 189457 known splicing junctions. 81111 novel splicing junctions.
sampling 10% (33282469) splicing reads. 331982 splicing junctions. 202847 known splicing junctions. 129135 novel splicing junctions.
sampling 15% (49923704) splicing reads. 375998 splicing junctions. 210001 known splicing junctions. 165997 novel splicing junctions.
sampling 20% (66564939) splicing reads. 410896 splicing junctions. 214793 known splicing junctions. 196103 novel splicing junctions.
sampling 25% (83206174) splicing reads. 439787 splicing junctions. 218237 known splicing junctions. 221550 novel splicing junctions.
sampling 30% (99847409) splicing reads. 464139 splicing junctions. 220902 known splicing junctions. 243237 novel splicing junctions.
sampling 35% (116488643) splicing reads. 485398 splicing junctions. 223141 known splicing junctions. 262257 novel splicing junctions.
sampling 40% (133129878) splicing reads. 503811 splicing junctions. 224948 known splicing junctions. 278863 novel splicing junctions.
sampling 45% (149771113) splicing reads. 520101 splicing junctions. 226411 known splicing junctions. 293690 novel splicing junctions.
sampling 50% (166412348) splicing reads. 534634 splicing junctions. 227655 known splicing junctions. 306979 novel splicing junctions.
sampling 55% (183053583) splicing reads. 547483 splicing junctions. 228751 known splicing junctions. 318732 novel splicing junctions.
sampling 60% (199694818) splicing reads. 559006 splicing junctions. 229660 known splicing junctions. 329346 novel splicing junctions.
sampling 65% (216336053) splicing reads. 569131 splicing junctions. 230439 known splicing junctions. 338692 novel splicing junctions.
sampling 70% (232977287) splicing reads. 578153 splicing junctions. 231118 known splicing junctions. 347035 novel splicing junctions.
sampling 75% (249618522) splicing reads. 586030 splicing junctions. 231693 known splicing junctions. 354337 novel splicing junctions.
sampling 80% (266259757) splicing reads. 593092 splicing junctions. 232251 known splicing junctions. 360841 novel splicing junctions.
sampling 85% (282900992) splicing reads. 599277 splicing junctions. 232711 known splicing junctions. 366566 novel splicing junctions.
sampling 90% (299542227) splicing reads. 604502 splicing junctions. 233094 known splicing junctions. 371408 novel splicing junctions.
sampling 95% (316183462) splicing reads. 608940 splicing junctions. 233405 known splicing junctions. 375535 novel splicing junctions.
sampling 100% (332824697) splicing reads. 612583 splicing junctions. 233694 known splicing junctions. 378889 novel splicing junctions.