File Info

Filename
qc_report_detailed.txt
Full Path
s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0006_exp0007/A23KKJ7LT4/nfcore_rnafusion__73d51683--20260607-225254/samplesheet_check/qc_report_detailed.txt
Size
18.8 KB
Published
Jun 08, 2026 12:00 AM
================================================================================
BCL CONVERT QC REPORT - DETAILED
================================================================================

1. % UNDETERMINED PER LANE
--------------------------------------------------------------------------------
      %_Undetermined  Undetermined_Reads  Total_Reads
Lane                                                 
1               4.66           201657180   4327821958
2               4.76           204854255   4304734692
3               4.83           209169038   4332980005
4               5.02           214139561   4267817260
5               5.28           224463395   4250454746
6               5.31           224448301   4229151809
7               5.32           227677607   4276187445
8               5.55           234654678   4228210849

2. SAMPLES WITH LOW READS (NON-NTC)
--------------------------------------------------------------------------------
No samples found with low reads

3. NTC SAMPLES WITH HIGH READS
--------------------------------------------------------------------------------
Found 2 NTC samples with high reads:
                    SampleID                  Index  # Reads
0  NTC_0001_0001_23KKJ7LT4_1  AGGCAATCGC-ATGAGTCTGT  6189071
1  NTC_0001_0001_23KKJ7LT4_2  GCATCCTACT-GACATCGCGA  5082195

4. TOP UNKNOWN BARCODES
--------------------------------------------------------------------------------
Found 138 significant unknown barcodes:
     Lane       index      index2   # Reads  % of Unknown Barcodes  % of All Reads  Percentage_of_Lane  Percentage_of_Total
0       1  CCAGTGCACT  GGGGGGGGGG  17071524               0.084656        0.003945            0.394460             0.049891
1       1  TTGGATTGTC  GGGGGGGGGG   9450316               0.046863        0.002184            0.218362             0.027618
2       1  AATTGGCACA  GGGGGGGGGG   6591205               0.032685        0.001523            0.152298             0.019263
3       1  TGCAACACTA  GGGGGGGGGG   6052821               0.030015        0.001399            0.139858             0.017689
4       1  TCGCATGACA  GGGGGGGGGG   4657991               0.023099        0.001076            0.107629             0.013613
5       1  GAAGCAAGCG  GGGGGGGGGG   4287288               0.021260        0.000991            0.099063             0.012530
6       1  ACAATAGCAC  GGGGGGGGGG   3821687               0.018951        0.000883            0.088305             0.011169
7       1  CCACCTGCTT  GGGGGGGGGG   3593237               0.017819        0.000830            0.083026             0.010501
8       1  GTACGCATCG  GGGGGGGGGG   3517495               0.017443        0.000813            0.081276             0.010280
9       1  CGTACTTAGA  GGGGGGGGGG   3034993               0.015050        0.000701            0.070127             0.008870
10      1  GATCTGTATG  GGGGGGGGGG   2345695               0.011632        0.000542            0.054200             0.006855
11      1  TTACGGACGC  GGGGGGGGGG   2312243               0.011466        0.000534            0.053427             0.006758
12      1  ACTTCCGTTC  GGGGGGGGGG   2254874               0.011182        0.000521            0.052102             0.006590
13      1  CATCAGGCGT  GGGGGGGGGG   2216465               0.010991        0.000512            0.051214             0.006478
14      2  CCAGTGCACT  GGGGGGGGGG  15691485               0.076598        0.003645            0.364517             0.045858
15      2  TTGGATTGTC  GGGGGGGGGG   8798536               0.042950        0.002044            0.204392             0.025714
16      2  AATTGGCACA  GGGGGGGGGG   6256110               0.030539        0.001453            0.145331             0.018283
17      2  TGCAACACTA  GGGGGGGGGG   5828771               0.028453        0.001354            0.135404             0.017035
18      2  TCGCATGACA  GGGGGGGGGG   4772549               0.023297        0.001109            0.110867             0.013948
19      2  GAAGCAAGCG  GGGGGGGGGG   3884918               0.018964        0.000902            0.090248             0.011354
20      2  ACAATAGCAC  GGGGGGGGGG   3704982               0.018086        0.000861            0.086068             0.010828
21      2  CCACCTGCTT  GGGGGGGGGG   3698399               0.018054        0.000859            0.085915             0.010809
22      2  GTACGCATCG  GGGGGGGGGG   3399116               0.016593        0.000790            0.078962             0.009934
23      2  CGTACTTAGA  GGGGGGGGGG   2858804               0.013955        0.000664            0.066411             0.008355
24      2  GATCTGTATG  GGGGGGGGGG   2495807               0.012183        0.000580            0.057978             0.007294
25      2  TTACGGACGC  GGGGGGGGGG   2429654               0.011860        0.000564            0.056441             0.007101
26      2  ACTTCCGTTC  GGGGGGGGGG   2324597               0.011348        0.000540            0.054001             0.006794
27      2  AGTACAACTG  GGGGGGGGGG   2252124               0.010994        0.000523            0.052317             0.006582
28      2  CTACAAGTTC  GGGGGGGGGG   2158134               0.010535        0.000501            0.050134             0.006307
29      3  CCAGTGCACT  GGGGGGGGGG  15180901               0.072577        0.003504            0.350357             0.044366
30      3  TTGGATTGTC  GGGGGGGGGG   8574303               0.040992        0.001979            0.197885             0.025058
31      3  AATTGGCACA  GGGGGGGGGG   6180787               0.029549        0.001426            0.142645             0.018063
32      3  TGCAACACTA  GGGGGGGGGG   5881459               0.028118        0.001357            0.135737             0.017189
33      3  TCGCATGACA  GGGGGGGGGG   4950297               0.023666        0.001142            0.114247             0.014467
34      3  GAAGCAAGCG  GGGGGGGGGG   3987420               0.019063        0.000920            0.092025             0.011653
35      3  CCACCTGCTT  GGGGGGGGGG   3890082               0.018598        0.000898            0.089778             0.011369
36      3  ACAATAGCAC  GGGGGGGGGG   3790102               0.018120        0.000875            0.087471             0.011077
37      3  GTACGCATCG  GGGGGGGGGG   3531683               0.016884        0.000815            0.081507             0.010321
38      3  CGTACTTAGA  GGGGGGGGGG   3094222               0.014793        0.000714            0.071411             0.009043
39      3  GATCTGTATG  GGGGGGGGGG   2591359               0.012389        0.000598            0.059805             0.007573
40      3  TTACGGACGC  GGGGGGGGGG   2511405               0.012007        0.000580            0.057960             0.007340
41      3  ACTTCCGTTC  GGGGGGGGGG   2445214               0.011690        0.000564            0.056433             0.007146
42      3  AGTACAACTG  GGGGGGGGGG   2394711               0.011449        0.000553            0.055267             0.006999
43      3  CTACAAGTTC  GGGGGGGGGG   2273212               0.010868        0.000525            0.052463             0.006643
44      4  CCAGTGCACT  GGGGGGGGGG  14615746               0.068253        0.003425            0.342464             0.042714
45      4  TTGGATTGTC  GGGGGGGGGG   8311691               0.038814        0.001948            0.194753             0.024291
46      4  AATTGGCACA  GGGGGGGGGG   6220867               0.029051        0.001458            0.145762             0.018180
47      4  TGCAACACTA  GGGGGGGGGG   6095722               0.028466        0.001428            0.142830             0.017815
48      4  TCGCATGACA  GGGGGGGGGG   5016570               0.023427        0.001175            0.117544             0.014661
49      4  GAAGCAAGCG  GGGGGGGGGG   4337469               0.020255        0.001016            0.101632             0.012676
50      4  CCACCTGCTT  GGGGGGGGGG   4094555               0.019121        0.000959            0.095940             0.011966
51      4  ACAATAGCAC  GGGGGGGGGG   3829519               0.017883        0.000897            0.089730             0.011192
52      4  GTACGCATCG  GGGGGGGGGG   3712787               0.017338        0.000870            0.086995             0.010851
53      4  CGTACTTAGA  GGGGGGGGGG   3433570               0.016034        0.000805            0.080453             0.010035
54      4  GATCTGTATG  GGGGGGGGGG   2995399               0.013988        0.000702            0.070186             0.008754
55      4  ACTTCCGTTC  GGGGGGGGGG   2723886               0.012720        0.000638            0.063824             0.007961
56      4  TTACGGACGC  GGGGGGGGGG   2714440               0.012676        0.000636            0.063603             0.007933
57      4  AGTACAACTG  GGGGGGGGGG   2664015               0.012441        0.000624            0.062421             0.007786
58      4  CTACAAGTTC  GGGGGGGGGG   2471397               0.011541        0.000579            0.057908             0.007223
59      4  ATAGGCAGTG  GGGGGGGGGG   2264720               0.010576        0.000531            0.053065             0.006619
60      4  TCAATCTAGC  GGGGGGGGGG   2260632               0.010557        0.000530            0.052969             0.006607
61      4  GATGCTAGAA  GGGGGGGGGG   2210396               0.010322        0.000518            0.051792             0.006460
62      5  CTCACCAGTT  GGGGGGGGGG   9482600               0.042246        0.002231            0.223096             0.027713
63      5  AATAGACTGC  GGGGGGGGGG   8739035               0.038933        0.002056            0.205602             0.025540
64      5  TAGAAGATCG  GGGGGGGGGG   7612531               0.033914        0.001791            0.179099             0.022248
65      5  AGTCCAGCGT  GGGGGGGGGG   7336649               0.032685        0.001726            0.172609             0.021441
66      5  AGGACGGACA  GGGGGGGGGG   7140315               0.031811        0.001680            0.167989             0.020868
67      5  CGGCATATAC  GGGGGGGGGG   4696827               0.020925        0.001105            0.110502             0.013726
68      5  CAACGTTGAC  GGGGGGGGGG   4332297               0.019301        0.001019            0.101925             0.012661
69      5  CATCACCTAA  GGGGGGGGGG   3703435               0.016499        0.000871            0.087130             0.010823
70      5  CCTGGAATTA  GGGGGGGGGG   3293110               0.014671        0.000775            0.077477             0.009624
71      5  ACGGTCGCAT  CGTCATCGAA   3259393               0.014521        0.000767            0.076683             0.009526
72      5  CCTATGTGTA  GGGGGGGGGG   3118628               0.013894        0.000734            0.073372             0.009114
73      5  GCGCGGTTAA  GGGGGGGGGG   2969540               0.013230        0.000699            0.069864             0.008678
74      5  GCGAAGTAGG  CAGGCCATGT   2885608               0.012856        0.000679            0.067889             0.008433
75      5  GCGACTCGTA  GGGGGGGGGG   2876524               0.012815        0.000677            0.067676             0.008407
76      5  GCGCGGTTAA  GTACTCTCTA   2851901               0.012705        0.000671            0.067096             0.008335
77      5  GCGATGGAAG  GGGGGGGGGG   2763468               0.012311        0.000650            0.065016             0.008076
78      5  ACGGTCGCAT  AAGCTATAGC   2747653               0.012241        0.000646            0.064644             0.008030
79      5  CACGAGTGTC  GGGGGGGGGG   2679571               0.011938        0.000630            0.063042             0.007831
80      5  ACAGAGGCGT  GGGGGGGGGG   2389090               0.010644        0.000562            0.056208             0.006982
81      6  CTCACCAGTT  GGGGGGGGGG   9976890               0.044451        0.002359            0.235908             0.029157
82      6  AATAGACTGC  GGGGGGGGGG   8859001               0.039470        0.002095            0.209475             0.025890
83      6  TAGAAGATCG  GGGGGGGGGG   8279894               0.036890        0.001958            0.195781             0.024198
84      6  AGTCCAGCGT  GGGGGGGGGG   7888127               0.035145        0.001865            0.186518             0.023053
85      6  AGGACGGACA  GGGGGGGGGG   7882113               0.035118        0.001864            0.186376             0.023035
86      6  CGGCATATAC  GGGGGGGGGG   4700725               0.020943        0.001112            0.111151             0.013738
87      6  CAACGTTGAC  GGGGGGGGGG   4295625               0.019139        0.001016            0.101572             0.012554
88      6  CATCACCTAA  GGGGGGGGGG   3757044               0.016739        0.000888            0.088837             0.010980
89      6  CCTGGAATTA  GGGGGGGGGG   3345085               0.014904        0.000791            0.079096             0.009776
90      6  ACGGTCGCAT  CGTCATCGAA   3322547               0.014803        0.000786            0.078563             0.009710
91      6  CCTATGTGTA  GGGGGGGGGG   2908761               0.012960        0.000688            0.068779             0.008501
92      6  GCGACTCGTA  GGGGGGGGGG   2862085               0.012752        0.000677            0.067675             0.008364
93      6  GCGCGGTTAA  GTACTCTCTA   2842978               0.012667        0.000672            0.067223             0.008309
94      6  GCGCGGTTAA  GGGGGGGGGG   2832696               0.012621        0.000670            0.066980             0.008279
95      6  GCGAAGTAGG  CAGGCCATGT   2827767               0.012599        0.000669            0.066864             0.008264
96      6  ACGGTCGCAT  AAGCTATAGC   2787528               0.012419        0.000659            0.065912             0.008147
97      6  GCGATGGAAG  GGGGGGGGGG   2677435               0.011929        0.000633            0.063309             0.007825
98      6  CACGAGTGTC  GGGGGGGGGG   2521842               0.011236        0.000596            0.059630             0.007370
99      6  ACAGAGGCGT  GGGGGGGGGG   2308460               0.010285        0.000546            0.054584             0.006746
100     7  CTCACCAGTT  GGGGGGGGGG  10273297               0.045122        0.002402            0.240244             0.030024
101     7  AATAGACTGC  GGGGGGGGGG   9223699               0.040512        0.002157            0.215699             0.026956
102     7  TAGAAGATCG  GGGGGGGGGG   8675847               0.038106        0.002029            0.202887             0.025355
103     7  AGTCCAGCGT  GGGGGGGGGG   8261550               0.036286        0.001932            0.193199             0.024144
104     7  AGGACGGACA  GGGGGGGGGG   8058779               0.035396        0.001885            0.188457             0.023552
105     7  CGGCATATAC  GGGGGGGGGG   4766496               0.020935        0.001115            0.111466             0.013930
106     7  CAACGTTGAC  GGGGGGGGGG   4356298               0.019134        0.001019            0.101873             0.012731
107     7  CATCACCTAA  GGGGGGGGGG   3820382               0.016780        0.000893            0.089341             0.011165
108     7  CCTGGAATTA  GGGGGGGGGG   3541234               0.015554        0.000828            0.082813             0.010349
109     7  ACGGTCGCAT  CGTCATCGAA   3349088               0.014710        0.000783            0.078319             0.009788
110     7  GCGACTCGTA  GGGGGGGGGG   2970326               0.013046        0.000695            0.069462             0.008681
111     7  GCGCGGTTAA  GGGGGGGGGG   2955530               0.012981        0.000691            0.069116             0.008638
112     7  CCTATGTGTA  GGGGGGGGGG   2933578               0.012885        0.000686            0.068603             0.008573
113     7  GCGCGGTTAA  GTACTCTCTA   2868979               0.012601        0.000671            0.067092             0.008385
114     7  GCGAAGTAGG  CAGGCCATGT   2868818               0.012600        0.000671            0.067088             0.008384
115     7  GCGATGGAAG  GGGGGGGGGG   2862104               0.012571        0.000669            0.066931             0.008364
116     7  ACGGTCGCAT  AAGCTATAGC   2806545               0.012327        0.000656            0.065632             0.008202
117     7  CACGAGTGTC  GGGGGGGGGG   2614985               0.011485        0.000612            0.061152             0.007642
118     7  ACAGAGGCGT  GGGGGGGGGG   2326372               0.010218        0.000544            0.054403             0.006799
119     8  CTCACCAGTT  GGGGGGGGGG  10762895               0.045867        0.002545            0.254550             0.031454
120     8  AATAGACTGC  GGGGGGGGGG  10077705               0.042947        0.002383            0.238344             0.029452
121     8  TAGAAGATCG  GGGGGGGGGG   9043631               0.038540        0.002139            0.213888             0.026430
122     8  AGTCCAGCGT  GGGGGGGGGG   8606056               0.036675        0.002035            0.203539             0.025151
123     8  AGGACGGACA  GGGGGGGGGG   8248868               0.035153        0.001951            0.195091             0.024107
124     8  CGGCATATAC  GGGGGGGGGG   4920299               0.020968        0.001164            0.116368             0.014380
125     8  CAACGTTGAC  GGGGGGGGGG   4651706               0.019824        0.001100            0.110016             0.013595
126     8  CATCACCTAA  GGGGGGGGGG   4109300               0.017512        0.000972            0.097188             0.012009
127     8  CCTGGAATTA  GGGGGGGGGG   3652859               0.015567        0.000864            0.086393             0.010675
128     8  ACGGTCGCAT  CGTCATCGAA   3290300               0.014022        0.000778            0.077818             0.009616
129     8  CCTATGTGTA  GGGGGGGGGG   3157234               0.013455        0.000747            0.074671             0.009227
130     8  GCGACTCGTA  GGGGGGGGGG   3068052               0.013075        0.000726            0.072561             0.008966
131     8  GCGCGGTTAA  GGGGGGGGGG   3047624               0.012988        0.000721            0.072078             0.008907
132     8  GCGATGGAAG  GGGGGGGGGG   2975291               0.012679        0.000704            0.070368             0.008695
133     8  GCGCGGTTAA  GTACTCTCTA   2835885               0.012085        0.000671            0.067071             0.008288
134     8  GCGAAGTAGG  CAGGCCATGT   2832434               0.012071        0.000670            0.066989             0.008278
135     8  ACGGTCGCAT  AAGCTATAGC   2752574               0.011730        0.000651            0.065100             0.008044
136     8  CACGAGTGTC  GGGGGGGGGG   2728874               0.011629        0.000645            0.064540             0.007975
137     8  ACAGAGGCGT  GGGGGGGGGG   2403712               0.010244        0.000568            0.056849             0.007025

5. BARCODES WITH HIGH MISMATCH RATES
--------------------------------------------------------------------------------
No barcodes found with high mismatch rates

================================================================================
FINAL STATUS CHECK
================================================================================
Overall % Undetermined: 5.09%
Status: FAIL
Reason(s):
  Overall undetermined 5.09% >= 5.0%
  NTC sample NTC_0001_0001_23KKJ7LT4_1 has 6,189,071 reads > 2,000,000
  NTC sample NTC_0001_0001_23KKJ7LT4_2 has 5,082,195 reads > 2,000,000