================================================================================ BCL CONVERT QC REPORT - DETAILED ================================================================================ 1. % UNDETERMINED PER LANE -------------------------------------------------------------------------------- %_Undetermined Undetermined_Reads Total_Reads Lane 1 4.66 201657180 4327821958 2 4.76 204854255 4304734692 3 4.83 209169038 4332980005 4 5.02 214139561 4267817260 5 5.28 224463395 4250454746 6 5.31 224448301 4229151809 7 5.32 227677607 4276187445 8 5.55 234654678 4228210849 2. SAMPLES WITH LOW READS (NON-NTC) -------------------------------------------------------------------------------- No samples found with low reads 3. NTC SAMPLES WITH HIGH READS -------------------------------------------------------------------------------- Found 2 NTC samples with high reads: SampleID Index # Reads 0 NTC_0001_0001_23KKJ7LT4_1 AGGCAATCGC-ATGAGTCTGT 6189071 1 NTC_0001_0001_23KKJ7LT4_2 GCATCCTACT-GACATCGCGA 5082195 4. TOP UNKNOWN BARCODES -------------------------------------------------------------------------------- Found 138 significant unknown barcodes: Lane index index2 # Reads % of Unknown Barcodes % of All Reads Percentage_of_Lane Percentage_of_Total 0 1 CCAGTGCACT GGGGGGGGGG 17071524 0.084656 0.003945 0.394460 0.049891 1 1 TTGGATTGTC GGGGGGGGGG 9450316 0.046863 0.002184 0.218362 0.027618 2 1 AATTGGCACA GGGGGGGGGG 6591205 0.032685 0.001523 0.152298 0.019263 3 1 TGCAACACTA GGGGGGGGGG 6052821 0.030015 0.001399 0.139858 0.017689 4 1 TCGCATGACA GGGGGGGGGG 4657991 0.023099 0.001076 0.107629 0.013613 5 1 GAAGCAAGCG GGGGGGGGGG 4287288 0.021260 0.000991 0.099063 0.012530 6 1 ACAATAGCAC GGGGGGGGGG 3821687 0.018951 0.000883 0.088305 0.011169 7 1 CCACCTGCTT GGGGGGGGGG 3593237 0.017819 0.000830 0.083026 0.010501 8 1 GTACGCATCG GGGGGGGGGG 3517495 0.017443 0.000813 0.081276 0.010280 9 1 CGTACTTAGA GGGGGGGGGG 3034993 0.015050 0.000701 0.070127 0.008870 10 1 GATCTGTATG GGGGGGGGGG 2345695 0.011632 0.000542 0.054200 0.006855 11 1 TTACGGACGC GGGGGGGGGG 2312243 0.011466 0.000534 0.053427 0.006758 12 1 ACTTCCGTTC GGGGGGGGGG 2254874 0.011182 0.000521 0.052102 0.006590 13 1 CATCAGGCGT GGGGGGGGGG 2216465 0.010991 0.000512 0.051214 0.006478 14 2 CCAGTGCACT GGGGGGGGGG 15691485 0.076598 0.003645 0.364517 0.045858 15 2 TTGGATTGTC GGGGGGGGGG 8798536 0.042950 0.002044 0.204392 0.025714 16 2 AATTGGCACA GGGGGGGGGG 6256110 0.030539 0.001453 0.145331 0.018283 17 2 TGCAACACTA GGGGGGGGGG 5828771 0.028453 0.001354 0.135404 0.017035 18 2 TCGCATGACA GGGGGGGGGG 4772549 0.023297 0.001109 0.110867 0.013948 19 2 GAAGCAAGCG GGGGGGGGGG 3884918 0.018964 0.000902 0.090248 0.011354 20 2 ACAATAGCAC GGGGGGGGGG 3704982 0.018086 0.000861 0.086068 0.010828 21 2 CCACCTGCTT GGGGGGGGGG 3698399 0.018054 0.000859 0.085915 0.010809 22 2 GTACGCATCG GGGGGGGGGG 3399116 0.016593 0.000790 0.078962 0.009934 23 2 CGTACTTAGA GGGGGGGGGG 2858804 0.013955 0.000664 0.066411 0.008355 24 2 GATCTGTATG GGGGGGGGGG 2495807 0.012183 0.000580 0.057978 0.007294 25 2 TTACGGACGC GGGGGGGGGG 2429654 0.011860 0.000564 0.056441 0.007101 26 2 ACTTCCGTTC GGGGGGGGGG 2324597 0.011348 0.000540 0.054001 0.006794 27 2 AGTACAACTG GGGGGGGGGG 2252124 0.010994 0.000523 0.052317 0.006582 28 2 CTACAAGTTC GGGGGGGGGG 2158134 0.010535 0.000501 0.050134 0.006307 29 3 CCAGTGCACT GGGGGGGGGG 15180901 0.072577 0.003504 0.350357 0.044366 30 3 TTGGATTGTC GGGGGGGGGG 8574303 0.040992 0.001979 0.197885 0.025058 31 3 AATTGGCACA GGGGGGGGGG 6180787 0.029549 0.001426 0.142645 0.018063 32 3 TGCAACACTA GGGGGGGGGG 5881459 0.028118 0.001357 0.135737 0.017189 33 3 TCGCATGACA GGGGGGGGGG 4950297 0.023666 0.001142 0.114247 0.014467 34 3 GAAGCAAGCG GGGGGGGGGG 3987420 0.019063 0.000920 0.092025 0.011653 35 3 CCACCTGCTT GGGGGGGGGG 3890082 0.018598 0.000898 0.089778 0.011369 36 3 ACAATAGCAC GGGGGGGGGG 3790102 0.018120 0.000875 0.087471 0.011077 37 3 GTACGCATCG GGGGGGGGGG 3531683 0.016884 0.000815 0.081507 0.010321 38 3 CGTACTTAGA GGGGGGGGGG 3094222 0.014793 0.000714 0.071411 0.009043 39 3 GATCTGTATG GGGGGGGGGG 2591359 0.012389 0.000598 0.059805 0.007573 40 3 TTACGGACGC GGGGGGGGGG 2511405 0.012007 0.000580 0.057960 0.007340 41 3 ACTTCCGTTC GGGGGGGGGG 2445214 0.011690 0.000564 0.056433 0.007146 42 3 AGTACAACTG GGGGGGGGGG 2394711 0.011449 0.000553 0.055267 0.006999 43 3 CTACAAGTTC GGGGGGGGGG 2273212 0.010868 0.000525 0.052463 0.006643 44 4 CCAGTGCACT GGGGGGGGGG 14615746 0.068253 0.003425 0.342464 0.042714 45 4 TTGGATTGTC GGGGGGGGGG 8311691 0.038814 0.001948 0.194753 0.024291 46 4 AATTGGCACA GGGGGGGGGG 6220867 0.029051 0.001458 0.145762 0.018180 47 4 TGCAACACTA GGGGGGGGGG 6095722 0.028466 0.001428 0.142830 0.017815 48 4 TCGCATGACA GGGGGGGGGG 5016570 0.023427 0.001175 0.117544 0.014661 49 4 GAAGCAAGCG GGGGGGGGGG 4337469 0.020255 0.001016 0.101632 0.012676 50 4 CCACCTGCTT GGGGGGGGGG 4094555 0.019121 0.000959 0.095940 0.011966 51 4 ACAATAGCAC GGGGGGGGGG 3829519 0.017883 0.000897 0.089730 0.011192 52 4 GTACGCATCG GGGGGGGGGG 3712787 0.017338 0.000870 0.086995 0.010851 53 4 CGTACTTAGA GGGGGGGGGG 3433570 0.016034 0.000805 0.080453 0.010035 54 4 GATCTGTATG GGGGGGGGGG 2995399 0.013988 0.000702 0.070186 0.008754 55 4 ACTTCCGTTC GGGGGGGGGG 2723886 0.012720 0.000638 0.063824 0.007961 56 4 TTACGGACGC GGGGGGGGGG 2714440 0.012676 0.000636 0.063603 0.007933 57 4 AGTACAACTG GGGGGGGGGG 2664015 0.012441 0.000624 0.062421 0.007786 58 4 CTACAAGTTC GGGGGGGGGG 2471397 0.011541 0.000579 0.057908 0.007223 59 4 ATAGGCAGTG GGGGGGGGGG 2264720 0.010576 0.000531 0.053065 0.006619 60 4 TCAATCTAGC GGGGGGGGGG 2260632 0.010557 0.000530 0.052969 0.006607 61 4 GATGCTAGAA GGGGGGGGGG 2210396 0.010322 0.000518 0.051792 0.006460 62 5 CTCACCAGTT GGGGGGGGGG 9482600 0.042246 0.002231 0.223096 0.027713 63 5 AATAGACTGC GGGGGGGGGG 8739035 0.038933 0.002056 0.205602 0.025540 64 5 TAGAAGATCG GGGGGGGGGG 7612531 0.033914 0.001791 0.179099 0.022248 65 5 AGTCCAGCGT GGGGGGGGGG 7336649 0.032685 0.001726 0.172609 0.021441 66 5 AGGACGGACA GGGGGGGGGG 7140315 0.031811 0.001680 0.167989 0.020868 67 5 CGGCATATAC GGGGGGGGGG 4696827 0.020925 0.001105 0.110502 0.013726 68 5 CAACGTTGAC GGGGGGGGGG 4332297 0.019301 0.001019 0.101925 0.012661 69 5 CATCACCTAA GGGGGGGGGG 3703435 0.016499 0.000871 0.087130 0.010823 70 5 CCTGGAATTA GGGGGGGGGG 3293110 0.014671 0.000775 0.077477 0.009624 71 5 ACGGTCGCAT CGTCATCGAA 3259393 0.014521 0.000767 0.076683 0.009526 72 5 CCTATGTGTA GGGGGGGGGG 3118628 0.013894 0.000734 0.073372 0.009114 73 5 GCGCGGTTAA GGGGGGGGGG 2969540 0.013230 0.000699 0.069864 0.008678 74 5 GCGAAGTAGG CAGGCCATGT 2885608 0.012856 0.000679 0.067889 0.008433 75 5 GCGACTCGTA GGGGGGGGGG 2876524 0.012815 0.000677 0.067676 0.008407 76 5 GCGCGGTTAA GTACTCTCTA 2851901 0.012705 0.000671 0.067096 0.008335 77 5 GCGATGGAAG GGGGGGGGGG 2763468 0.012311 0.000650 0.065016 0.008076 78 5 ACGGTCGCAT AAGCTATAGC 2747653 0.012241 0.000646 0.064644 0.008030 79 5 CACGAGTGTC GGGGGGGGGG 2679571 0.011938 0.000630 0.063042 0.007831 80 5 ACAGAGGCGT GGGGGGGGGG 2389090 0.010644 0.000562 0.056208 0.006982 81 6 CTCACCAGTT GGGGGGGGGG 9976890 0.044451 0.002359 0.235908 0.029157 82 6 AATAGACTGC GGGGGGGGGG 8859001 0.039470 0.002095 0.209475 0.025890 83 6 TAGAAGATCG GGGGGGGGGG 8279894 0.036890 0.001958 0.195781 0.024198 84 6 AGTCCAGCGT GGGGGGGGGG 7888127 0.035145 0.001865 0.186518 0.023053 85 6 AGGACGGACA GGGGGGGGGG 7882113 0.035118 0.001864 0.186376 0.023035 86 6 CGGCATATAC GGGGGGGGGG 4700725 0.020943 0.001112 0.111151 0.013738 87 6 CAACGTTGAC GGGGGGGGGG 4295625 0.019139 0.001016 0.101572 0.012554 88 6 CATCACCTAA GGGGGGGGGG 3757044 0.016739 0.000888 0.088837 0.010980 89 6 CCTGGAATTA GGGGGGGGGG 3345085 0.014904 0.000791 0.079096 0.009776 90 6 ACGGTCGCAT CGTCATCGAA 3322547 0.014803 0.000786 0.078563 0.009710 91 6 CCTATGTGTA GGGGGGGGGG 2908761 0.012960 0.000688 0.068779 0.008501 92 6 GCGACTCGTA GGGGGGGGGG 2862085 0.012752 0.000677 0.067675 0.008364 93 6 GCGCGGTTAA GTACTCTCTA 2842978 0.012667 0.000672 0.067223 0.008309 94 6 GCGCGGTTAA GGGGGGGGGG 2832696 0.012621 0.000670 0.066980 0.008279 95 6 GCGAAGTAGG CAGGCCATGT 2827767 0.012599 0.000669 0.066864 0.008264 96 6 ACGGTCGCAT AAGCTATAGC 2787528 0.012419 0.000659 0.065912 0.008147 97 6 GCGATGGAAG GGGGGGGGGG 2677435 0.011929 0.000633 0.063309 0.007825 98 6 CACGAGTGTC GGGGGGGGGG 2521842 0.011236 0.000596 0.059630 0.007370 99 6 ACAGAGGCGT GGGGGGGGGG 2308460 0.010285 0.000546 0.054584 0.006746 100 7 CTCACCAGTT GGGGGGGGGG 10273297 0.045122 0.002402 0.240244 0.030024 101 7 AATAGACTGC GGGGGGGGGG 9223699 0.040512 0.002157 0.215699 0.026956 102 7 TAGAAGATCG GGGGGGGGGG 8675847 0.038106 0.002029 0.202887 0.025355 103 7 AGTCCAGCGT GGGGGGGGGG 8261550 0.036286 0.001932 0.193199 0.024144 104 7 AGGACGGACA GGGGGGGGGG 8058779 0.035396 0.001885 0.188457 0.023552 105 7 CGGCATATAC GGGGGGGGGG 4766496 0.020935 0.001115 0.111466 0.013930 106 7 CAACGTTGAC GGGGGGGGGG 4356298 0.019134 0.001019 0.101873 0.012731 107 7 CATCACCTAA GGGGGGGGGG 3820382 0.016780 0.000893 0.089341 0.011165 108 7 CCTGGAATTA GGGGGGGGGG 3541234 0.015554 0.000828 0.082813 0.010349 109 7 ACGGTCGCAT CGTCATCGAA 3349088 0.014710 0.000783 0.078319 0.009788 110 7 GCGACTCGTA GGGGGGGGGG 2970326 0.013046 0.000695 0.069462 0.008681 111 7 GCGCGGTTAA GGGGGGGGGG 2955530 0.012981 0.000691 0.069116 0.008638 112 7 CCTATGTGTA GGGGGGGGGG 2933578 0.012885 0.000686 0.068603 0.008573 113 7 GCGCGGTTAA GTACTCTCTA 2868979 0.012601 0.000671 0.067092 0.008385 114 7 GCGAAGTAGG CAGGCCATGT 2868818 0.012600 0.000671 0.067088 0.008384 115 7 GCGATGGAAG GGGGGGGGGG 2862104 0.012571 0.000669 0.066931 0.008364 116 7 ACGGTCGCAT AAGCTATAGC 2806545 0.012327 0.000656 0.065632 0.008202 117 7 CACGAGTGTC GGGGGGGGGG 2614985 0.011485 0.000612 0.061152 0.007642 118 7 ACAGAGGCGT GGGGGGGGGG 2326372 0.010218 0.000544 0.054403 0.006799 119 8 CTCACCAGTT GGGGGGGGGG 10762895 0.045867 0.002545 0.254550 0.031454 120 8 AATAGACTGC GGGGGGGGGG 10077705 0.042947 0.002383 0.238344 0.029452 121 8 TAGAAGATCG GGGGGGGGGG 9043631 0.038540 0.002139 0.213888 0.026430 122 8 AGTCCAGCGT GGGGGGGGGG 8606056 0.036675 0.002035 0.203539 0.025151 123 8 AGGACGGACA GGGGGGGGGG 8248868 0.035153 0.001951 0.195091 0.024107 124 8 CGGCATATAC GGGGGGGGGG 4920299 0.020968 0.001164 0.116368 0.014380 125 8 CAACGTTGAC GGGGGGGGGG 4651706 0.019824 0.001100 0.110016 0.013595 126 8 CATCACCTAA GGGGGGGGGG 4109300 0.017512 0.000972 0.097188 0.012009 127 8 CCTGGAATTA GGGGGGGGGG 3652859 0.015567 0.000864 0.086393 0.010675 128 8 ACGGTCGCAT CGTCATCGAA 3290300 0.014022 0.000778 0.077818 0.009616 129 8 CCTATGTGTA GGGGGGGGGG 3157234 0.013455 0.000747 0.074671 0.009227 130 8 GCGACTCGTA GGGGGGGGGG 3068052 0.013075 0.000726 0.072561 0.008966 131 8 GCGCGGTTAA GGGGGGGGGG 3047624 0.012988 0.000721 0.072078 0.008907 132 8 GCGATGGAAG GGGGGGGGGG 2975291 0.012679 0.000704 0.070368 0.008695 133 8 GCGCGGTTAA GTACTCTCTA 2835885 0.012085 0.000671 0.067071 0.008288 134 8 GCGAAGTAGG CAGGCCATGT 2832434 0.012071 0.000670 0.066989 0.008278 135 8 ACGGTCGCAT AAGCTATAGC 2752574 0.011730 0.000651 0.065100 0.008044 136 8 CACGAGTGTC GGGGGGGGGG 2728874 0.011629 0.000645 0.064540 0.007975 137 8 ACAGAGGCGT GGGGGGGGGG 2403712 0.010244 0.000568 0.056849 0.007025 5. BARCODES WITH HIGH MISMATCH RATES -------------------------------------------------------------------------------- No barcodes found with high mismatch rates ================================================================================ FINAL STATUS CHECK ================================================================================ Overall % Undetermined: 5.09% Status: FAIL Reason(s): Overall undetermined 5.09% >= 5.0% NTC sample NTC_0001_0001_23KKJ7LT4_1 has 6,189,071 reads > 2,000,000 NTC sample NTC_0001_0001_23KKJ7LT4_2 has 5,082,195 reads > 2,000,000