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BCL CONVERT QC REPORT - DETAILED
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1. % UNDETERMINED PER LANE
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%_Undetermined Undetermined_Reads Total_Reads
Lane
1 6.80 268308801 3943473605
2 6.81 267758180 3932731736
3 6.99 271863886 3889634474
4 7.33 283228840 3865350905
5 6.77 266843082 3943517784
6 6.76 266199678 3935511793
7 6.65 265625658 3994328926
8 6.64 265680856 4002815434
2. SAMPLES WITH LOW READS (NON-NTC)
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Found 17 samples with low reads:
SampleID Index # Reads Lane(s)
65 659_dkd-T1-TRNA-1_B23MHV2LT4_1 AGACGACAGA-GAGCTGGAAT 184741 1,2,3,4
50 659_cuO-T1-TRNA-1_B23MHV2LT4_1 TTCTACCGGC-TACTCGTAAG 192558 5,6,7,8
77 659_egp-T1-TRNA-1_B23MHV2LT4_1 GCCAAGTAAC-TGCGTGATGA 197292 5,6,7,8
76 659_eeB-T1-TRNA-1_B23MHV2LT4_1 CACGTAAGAG-TCAATCCGCG 205713 5,6,7,8
69 659_e0S-T1-TRNA-1_B23MHV2LT4_1 GCTACAGGTC-TTATGCAGGA 233173 5,6,7,8
2 659_8h-T1-TRNA-1_B23MHV2LT4_1 TAAGGCTCAC-GACGTTACTT 285607 1,2,3,4
49 659_cq5-T1-TRNA-1_B23MHV2LT4_1 TGCTTCCAAG-CAGATGTTAC 292068 1,2,3,4
33 659_buj-T1-TRNA-1_B23MHV2LT4_1 AATCGAACTC-ACGAACCTTA 319430 5,6,7,8
35 659_cAa-T1-TRNA-1_B23MHV2LT4_1 CCAACTGTAT-GCGCTAGAGA 332597 1,2,3,4
58 659_dQk-T1-TRNA-1_B23MHV2LT4_1 AGAGTATGTG-AGATGGCTTC 353103 5,6,7,8
12 659_Wm-T1-TRNA-1_B23MHV2LT4_1 GCAGATGTAA-TACACGCCTC 364298 5,6,7,8
54 659_d0V-T1-TRNA-1_B23MHV2LT4_1 ACCAGGAATT-AGCATGGCAT 367467 1,2,3,4
53 659_d0M-T1-TRNA-1_B23MHV2LT4_1 CAATGCGTGT-ATGTTCGCGC 395877 1,2,3,4
72 659_eKx-T1-TRNA-1_B23MHV2LT4_1 CATATGGTGC-ACAGATCTCA 397977 1,2,3,4
14 659_b6o-T1-TRNA-1_B23MHV2LT4_1 TAGCGAGGCT-TTGCTCACGG 437890 1,2,3,4
10 659_R1-T1-TRNA-1_B23MHV2LT4_1 TGTCAGGCTT-GATTCCTTAC 500047 1,2,3,4
68 659_dwi-T1-TRNA-1_B23MHV2LT4_1 TGTGACGTAT-GCAGCGTTAG 543963 1,2,3,4
3. NTC SAMPLES WITH HIGH READS
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No NTC samples found with high reads
4. TOP UNKNOWN BARCODES
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Found 106 significant unknown barcodes:
Lane index index2 # Reads % of Unknown Barcodes % of All Reads Percentage_of_Lane Percentage_of_Total
0 1 GATAGGTGAA GGGGGGGGGG 9536564 0.035543 0.002418 0.241832 0.030268
1 1 GTGACGCACA GGGGGGGGGG 8854352 0.033001 0.002245 0.224532 0.028102
2 1 TGAGTGTACA GGGGGGGGGG 8433102 0.031431 0.002138 0.213850 0.026765
3 1 AAGGTGTACT GGGGGGGGGG 7877642 0.029360 0.001998 0.199764 0.025003
4 1 ATCATACCGC GGGGGGGGGG 4719489 0.017590 0.001197 0.119678 0.014979
5 1 CTCTATACTG GGGGGGGGGG 4123997 0.015370 0.001046 0.104578 0.013089
6 1 CCTACTCTGC GGGGGGGGGG 3861447 0.014392 0.000979 0.097920 0.012256
7 1 TCTCCGTACT GGGGGGGGGG 3505481 0.013065 0.000889 0.088893 0.011126
8 1 TCGACCAGCA GGGGGGGGGG 3310068 0.012337 0.000839 0.083938 0.010506
9 1 GCACTACCAG GGGGGGGGGG 2076965 0.007741 0.000527 0.052668 0.006592
10 1 CCAGTATTCT GGGGGGGGGG 2026912 0.007554 0.000514 0.051399 0.006433
11 2 GATAGGTGAA GGGGGGGGGG 9212694 0.034407 0.002343 0.234257 0.029240
12 2 GTGACGCACA GGGGGGGGGG 8670621 0.032382 0.002205 0.220473 0.027519
13 2 TGAGTGTACA GGGGGGGGGG 8198443 0.030619 0.002085 0.208467 0.026021
14 2 AAGGTGTACT GGGGGGGGGG 7805231 0.029150 0.001985 0.198468 0.024773
15 2 ATCATACCGC GGGGGGGGGG 4799451 0.017925 0.001220 0.122039 0.015233
16 2 CCTACTCTGC GGGGGGGGGG 4144878 0.015480 0.001054 0.105394 0.013155
17 2 CTCTATACTG GGGGGGGGGG 4125703 0.015408 0.001049 0.104907 0.013094
18 2 TCTCCGTACT GGGGGGGGGG 3564323 0.013312 0.000906 0.090632 0.011313
19 2 TCGACCAGCA GGGGGGGGGG 3379177 0.012620 0.000859 0.085924 0.010725
20 2 ATCCAGCATG AGATCTCGGT 2704367 0.010100 0.000688 0.068766 0.008583
21 2 GCACTACCAG GGGGGGGGGG 2024674 0.007562 0.000515 0.051483 0.006426
22 2 CCAGTATTCT GGGGGGGGGG 2015291 0.007527 0.000512 0.051244 0.006396
23 3 GATAGGTGAA GGGGGGGGGG 9163090 0.033705 0.002356 0.235577 0.029082
24 3 GTGACGCACA GGGGGGGGGG 8641701 0.031787 0.002222 0.222173 0.027428
25 3 TGAGTGTACA GGGGGGGGGG 8128023 0.029897 0.002090 0.208966 0.025797
26 3 AAGGTGTACT GGGGGGGGGG 7971737 0.029323 0.002049 0.204948 0.025301
27 3 ATCATACCGC GGGGGGGGGG 4756283 0.017495 0.001223 0.122281 0.015096
28 3 CCTACTCTGC GGGGGGGGGG 4168999 0.015335 0.001072 0.107182 0.013232
29 3 CTCTATACTG GGGGGGGGGG 4140416 0.015230 0.001064 0.106447 0.013141
30 3 TCTCCGTACT GGGGGGGGGG 3695847 0.013594 0.000950 0.095018 0.011730
31 3 TCGACCAGCA GGGGGGGGGG 3463055 0.012738 0.000890 0.089033 0.010991
32 3 ATCCAGCATG AGATCTCGGT 2343065 0.008619 0.000602 0.060239 0.007437
33 3 CCAGTATTCT GGGGGGGGGG 2140242 0.007872 0.000550 0.055024 0.006793
34 3 GCACTACCAG GGGGGGGGGG 2128689 0.007830 0.000547 0.054727 0.006756
35 4 GATAGGTGAA GGGGGGGGGG 9072898 0.032034 0.002347 0.234724 0.028796
36 4 GTGACGCACA GGGGGGGGGG 8906444 0.031446 0.002304 0.230417 0.028268
37 4 AAGGTGTACT GGGGGGGGGG 8596644 0.030352 0.002224 0.222403 0.027285
38 4 TGAGTGTACA GGGGGGGGGG 8036447 0.028374 0.002079 0.207910 0.025507
39 4 ATCATACCGC GGGGGGGGGG 4975903 0.017568 0.001287 0.128731 0.015793
40 4 CCTACTCTGC GGGGGGGGGG 4517119 0.015949 0.001169 0.116862 0.014337
41 4 CTCTATACTG GGGGGGGGGG 4401435 0.015540 0.001139 0.113869 0.013970
42 4 TCTCCGTACT GGGGGGGGGG 4057957 0.014327 0.001050 0.104983 0.012879
43 4 TCGACCAGCA GGGGGGGGGG 3622473 0.012790 0.000937 0.093717 0.011497
44 4 CCAGTATTCT GGGGGGGGGG 2487267 0.008782 0.000643 0.064348 0.007894
45 4 GCACTACCAG GGGGGGGGGG 2408213 0.008503 0.000623 0.062303 0.007643
46 4 ATCCAGCATG AGATCTCGGT 2154935 0.007608 0.000558 0.055750 0.006839
47 5 GTCGACTCCT GGGGGGGGGG 13563411 0.050829 0.003439 0.343942 0.043048
48 5 ACAAGAACCT GGGGGGGGGG 7158402 0.026826 0.001815 0.181523 0.022720
49 5 CACGAGAAGT GGGGGGGGGG 6316072 0.023670 0.001602 0.160163 0.020046
50 5 ACTTAGATCG GGGGGGGGGG 4668609 0.017496 0.001184 0.118387 0.014818
51 5 TTGTGAAGGC GGGGGGGGGG 4640329 0.017390 0.001177 0.117670 0.014728
52 5 TTGCAATGCG GGGGGGGGGG 3858575 0.014460 0.000978 0.097846 0.012247
53 5 CCTCTGTCAT GGGGGGGGGG 3789823 0.014202 0.000961 0.096103 0.012028
54 5 ACCTTCGGAC GGGGGGGGGG 3722862 0.013952 0.000944 0.094405 0.011816
55 5 CATTATCGCT GGGGGGGGGG 3641882 0.013648 0.000924 0.092351 0.011559
56 5 CTTCGGAATC GGGGGGGGGG 2933468 0.010993 0.000744 0.074387 0.009310
57 5 AACATAAGGC GGGGGGGGGG 2733174 0.010243 0.000693 0.069308 0.008675
58 5 GATCAACAAG GGGGGGGGGG 2713155 0.010168 0.000688 0.068800 0.008611
59 5 CAACCATACA GGGGGGGGGG 2545846 0.009541 0.000646 0.064558 0.008080
60 5 ACTTCAACAG GGGGGGGGGG 2240273 0.008395 0.000568 0.056809 0.007110
61 5 CGGATTGATC GGGGGGGGGG 2103137 0.007882 0.000533 0.053331 0.006675
62 6 GTCGACTCCT GGGGGGGGGG 14936519 0.056110 0.003795 0.379532 0.047406
63 6 ACAAGAACCT GGGGGGGGGG 6972116 0.026191 0.001772 0.177159 0.022129
64 6 CACGAGAAGT GGGGGGGGGG 6526625 0.024518 0.001658 0.165839 0.020715
65 6 TTGTGAAGGC GGGGGGGGGG 4810088 0.018069 0.001222 0.122223 0.015267
66 6 ACTTAGATCG GGGGGGGGGG 4627079 0.017382 0.001176 0.117572 0.014686
67 6 ACCTTCGGAC GGGGGGGGGG 3929895 0.014763 0.000999 0.099857 0.012473
68 6 CCTCTGTCAT GGGGGGGGGG 3840607 0.014428 0.000976 0.097589 0.012190
69 6 TTGCAATGCG GGGGGGGGGG 3757990 0.014117 0.000955 0.095489 0.011927
70 6 CATTATCGCT GGGGGGGGGG 3756470 0.014111 0.000955 0.095451 0.011923
71 6 CTTCGGAATC GGGGGGGGGG 3112078 0.011691 0.000791 0.079077 0.009877
72 6 GATCAACAAG GGGGGGGGGG 2677814 0.010059 0.000680 0.068042 0.008499
73 6 AACATAAGGC GGGGGGGGGG 2608361 0.009799 0.000663 0.066278 0.008279
74 6 CAACCATACA GGGGGGGGGG 2389463 0.008976 0.000607 0.060715 0.007584
75 6 ACTTCAACAG GGGGGGGGGG 2038223 0.007657 0.000518 0.051791 0.006469
76 6 CGGATTGATC GGGGGGGGGG 2032622 0.007636 0.000516 0.051648 0.006451
77 7 GTCGACTCCT GGGGGGGGGG 15103196 0.056859 0.003781 0.378116 0.047935
78 7 ACAAGAACCT GGGGGGGGGG 7261921 0.027339 0.001818 0.181806 0.023048
79 7 CACGAGAAGT GGGGGGGGGG 6652969 0.025046 0.001666 0.166560 0.021116
80 7 TTGTGAAGGC GGGGGGGGGG 4948886 0.018631 0.001239 0.123898 0.015707
81 7 ACTTAGATCG GGGGGGGGGG 4800964 0.018074 0.001202 0.120195 0.015238
82 7 ACCTTCGGAC GGGGGGGGGG 4026177 0.015157 0.001008 0.100797 0.012779
83 7 CCTCTGTCAT GGGGGGGGGG 3900419 0.014684 0.000976 0.097649 0.012379
84 7 TTGCAATGCG GGGGGGGGGG 3892184 0.014653 0.000974 0.097443 0.012353
85 7 CATTATCGCT GGGGGGGGGG 3855487 0.014515 0.000965 0.096524 0.012237
86 7 CTTCGGAATC GGGGGGGGGG 3114668 0.011726 0.000780 0.077977 0.009886
87 7 GATCAACAAG GGGGGGGGGG 2770660 0.010431 0.000694 0.069365 0.008794
88 7 AACATAAGGC GGGGGGGGGG 2735297 0.010298 0.000685 0.068480 0.008681
89 7 CAACCATACA GGGGGGGGGG 2476809 0.009324 0.000620 0.062008 0.007861
90 7 ACTTCAACAG GGGGGGGGGG 2131175 0.008023 0.000534 0.053355 0.006764
91 7 CGGATTGATC GGGGGGGGGG 2130566 0.008021 0.000533 0.053340 0.006762
92 8 GTCGACTCCT GGGGGGGGGG 15533349 0.058466 0.003881 0.388061 0.049301
93 8 ACAAGAACCT GGGGGGGGGG 7270640 0.027366 0.001816 0.181638 0.023076
94 8 CACGAGAAGT GGGGGGGGGG 6913650 0.026022 0.001727 0.172720 0.021943
95 8 TTGTGAAGGC GGGGGGGGGG 5231938 0.019693 0.001307 0.130706 0.016605
96 8 ACTTAGATCG GGGGGGGGGG 4830318 0.018181 0.001207 0.120673 0.015331
97 8 ACCTTCGGAC GGGGGGGGGG 4053719 0.015258 0.001013 0.101272 0.012866
98 8 CCTCTGTCAT GGGGGGGGGG 3999048 0.015052 0.000999 0.099906 0.012692
99 8 TTGCAATGCG GGGGGGGGGG 3962933 0.014916 0.000990 0.099004 0.012578
100 8 CATTATCGCT GGGGGGGGGG 3819692 0.014377 0.000954 0.095425 0.012123
101 8 CTTCGGAATC GGGGGGGGGG 3232535 0.012167 0.000808 0.080757 0.010260
102 8 GATCAACAAG GGGGGGGGGG 2743122 0.010325 0.000685 0.068530 0.008706
103 8 AACATAAGGC GGGGGGGGGG 2706867 0.010188 0.000676 0.067624 0.008591
104 8 CAACCATACA GGGGGGGGGG 2366508 0.008907 0.000591 0.059121 0.007511
105 8 CGGATTGATC GGGGGGGGGG 2065660 0.007775 0.000516 0.051605 0.006556
5. BARCODES WITH HIGH MISMATCH RATES
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No barcodes found with high mismatch rates
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FINAL STATUS CHECK
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Overall % Undetermined: 6.84%
Status: WARNING
Reason(s):
Overall undetermined 6.84% >= 5.0%