FastQCFastQC Report
Thu 4 Jun 2026
659_8h-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_8h-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285607
Total Bases43.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCTAGT15792255.29346269524207TruSeq Adapter, Index 3 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCTGGT3198411.198605076206116TruSeq Adapter, Index 3 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCTAGG209067.319848603150484TruSeq Adapter, Index 3 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCTGGG154415.406380095725945TruSeq Adapter, Index 3 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCTAGTA26390.9239969608588025TruSeq Adapter, Index 3 (97% over 38bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA11340.39704909193402127No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG10990.38479449033111934No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCTAGTATG10320.36133568154842144TruSeq Adapter, Index 3 (97% over 36bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC8320.29130938667469636No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC6810.2384395340450339No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6330.22163322327533988No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCGAGT5340.18697020731284597TruSeq Adapter, Index 3 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCTGGTA5030.1761161316074186TruSeq Adapter, Index 3 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATATAGT4700.16456179295325396TruSeq Adapter, Index 3 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCGAGG3930.1376016694268698TruSeq Adapter, Index 3 (97% over 39bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3730.13059903993949729No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATGTAGT3600.12604733077270516TruSeq Adapter, Index 3 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCGGGT3450.12079535865717576TruSeq Adapter, Index 3 (97% over 39bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCTAGTA3250.11379272916980326TruSeq Adapter, Index 10 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)