FastQCFastQC Report
Thu 4 Jun 2026
659_Wm-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_Wm-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364298
Total Bases55 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGG17876049.06971764873812TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGT9020024.75994927229905TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTAGG180984.967910886142663TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTAGT144883.9769639141581896TruSeq Adapter, Index 5 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGGG29140.7998945917902376TruSeq Adapter, Index 5 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGTG10870.2983820937803666TruSeq Adapter, Index 5 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGGGTG8760.24046247852033226TruSeq Adapter, Index 5 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGTAAATCTGGG8450.23195296158639356TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCGGGG7960.2185024348198453TruSeq Adapter, Index 5 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGGGGG7320.20093439985945571TruSeq Adapter, Index 5 (97% over 34bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC5470.15015179880207963No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGTGTG5460.14987729825582352TruSeq Adapter, Index 5 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGTAAATCTGGT5250.1441127867844457TruSeq Adapter, Index 3 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA5220.14328928514567743No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGGA4960.13615227094301915TruSeq Adapter, Index 5 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATGTGGG4880.13395626657297047TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATTTGGG4790.1314857616566657TruSeq Adapter, Index 5 (97% over 37bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGCAGATCGGAAGAGCA4440.12187824253770264No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT4370.11995673871391004No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGTA4050.11117272123371524TruSeq Adapter, Index 5 (97% over 36bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGG4000.10980021850243481TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGGG3970.10897671686366656TruSeq Adapter, Index 6 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCGGGT3670.10074170047598395TruSeq Adapter, Index 5 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)