FastQCFastQC Report
Thu 4 Jun 2026
659_cq5-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_cq5-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292068
Total Bases44.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGG18088161.93112562827835TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGT4730316.195885889587355TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTAGG36361.2449155676075434TruSeq Adapter, Index 11 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGGG29241.0011367215853841TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTAGT19350.6625169481079748TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCGGGG12420.4252434364600025TruSeq Adapter, Index 11 (97% over 37bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA11120.38073325389977675No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG10710.36669542709232095No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGGA9960.34101647561526766TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATGTGGG8600.2944519769368777TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATTTGGG7160.2451483901009354TruSeq Adapter, Index 11 (97% over 37bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC7140.24446361806154732No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGGGTG6970.23864305572674857TruSeq Adapter, Index 11 (97% over 34bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA5550.19002424093019435No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC5440.18625799471355986No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGTG5200.1780407302409028TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATATGGG5060.1732473259651862TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCGGGT4140.14174781215333415TruSeq Adapter, Index 11 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGGGGG3570.12223180903077366TruSeq Adapter, Index 11 (97% over 34bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3330.1140145445581166No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGGG3120.10682443814454168TruSeq Adapter, Index 4 (96% over 30bp)
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGA3070.10511250804607147No Hit
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTCCAAGATCTGGG3060.10477012202637744TruSeq Adapter, Index 4 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)