FastQCFastQC Report
Thu 4 Jun 2026
659_d0V-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_d0V-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367467
Total Bases55.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTAGT20842256.71856248316175TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTGGT7086019.283364220460612TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTGGG78432.1343413150024357TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTAGG48721.3258333401366653TruSeq Adapter, Index 1 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTAGTA27680.7532649190267425TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTAGTG15890.4324197819123894TruSeq Adapter, Index 1 (97% over 36bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA14620.3978588553529977No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG13910.37853739247333773No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCGAGT11090.3017958075146884TruSeq Adapter, Index 1 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTAGTATG10390.28274647791502366TruSeq Adapter, Index 1 (97% over 34bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC10170.276759545755129No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTGGTG9800.2666906143953062TruSeq Adapter, Index 1 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA9260.2519954172755649No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCGGGT8690.23648382031583787TruSeq Adapter, Index 1 (97% over 37bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC8200.22314928959607255No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTGGTA6990.1902211627166521TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATATAGT6460.17579809887690595TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATGTAGT6290.17117183311698736TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATGTGGT5450.1483126375973897TruSeq Adapter, Index 1 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTAGTGTG5280.1436863718374711TruSeq Adapter, Index 1 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATTTAGT5140.13987650591753817TruSeq Adapter, Index 1 (97% over 37bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4960.13497810687762438No Hit
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATCTAGT4180.11375171103799797TruSeq Adapter, Index 5 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATTTGGT4110.11184677807803149TruSeq Adapter, Index 1 (97% over 37bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3850.10477131279815603No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGGAATTATATGGT3790.10313851311818475TruSeq Adapter, Index 1 (97% over 37bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAGATCGGAAGAGCAC3770.10259424655819434No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC3720.10123358015821829No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)