FastQCFastQC Report
Thu 4 Jun 2026
659_dQk-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_dQk-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353103
Total Bases53.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGG19032153.89957038031395TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGT6574418.618929887313335TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTAGG208895.915837588465688TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTAGT120763.4199652792527955TruSeq Adapter, Index 5 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGGG36161.024063800080996TruSeq Adapter, Index 5 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGGGGG13480.3817582971540882TruSeq Adapter, Index 5 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGTG10180.28830114725731587TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAAGAGTATGTGATCTGGG6820.19314477645332948TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGGGTG6740.1908791485770441TruSeq Adapter, Index 5 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6410.18153343358736684No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT6410.18153343358736684No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGGG4690.13282243424723097TruSeq Adapter, Index 13 (96% over 30bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGG4460.12630875410291048TruSeq Adapter, Index 13 (97% over 35bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4360.12347671925755374No Hit
GATCGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGGG4040.11441420775241218TruSeq Adapter, Index 5 (96% over 33bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC3900.11044935896891277No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTATGTGATCTGGTGTG3840.10875013806169873TruSeq Adapter, Index 5 (97% over 35bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3600.10195325443284255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)