FastQCFastQC Report
Thu 4 Jun 2026
659_dkd-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_dkd-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184741
Total Bases27.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC75

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTGGG10599457.37437818351096TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTAGG2936915.897391483211631TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTGGT35321.9118658013110246TruSeq Adapter, Index 4 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTGGGG21421.1594610833545342TruSeq Adapter, Index 4 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTAGT20711.1210288999193465TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCGGGG11350.6143736366047602TruSeq Adapter, Index 4 (97% over 37bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA10700.579189243319025No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG10370.5613263974970364No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTGGGGGG7790.4216714210705799TruSeq Adapter, Index 4 (97% over 34bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC7190.38919351957605514No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCGAGG5790.31341174942216404TruSeq Adapter, Index 4 (97% over 37bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC5480.2966315003166595No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATGTGGG5270.2852642347935759TruSeq Adapter, Index 4 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTAGGG4730.25603412344850357TruSeq Adapter, Index 4 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATATGGG3590.19432611060890653TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATTTGGG3470.18783053031000155TruSeq Adapter, Index 4 (97% over 37bp)
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGA3180.17213287792098125No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2990.16184820911438177No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA2730.1477744518000877No Hit
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTGGG2640.14290276657590897TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTGGGG2380.1288290092616149TruSeq Adapter, Index 13 (96% over 30bp)
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC2350.12720511418688868No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATGTAGG2340.1266638158286466TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATATAGG2300.12449862239567829TruSeq Adapter, Index 4 (97% over 37bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAGATCGGAAGAGCAC2170.11746174373853124No Hit
GATCGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATCTGGGG2120.11475525194732084TruSeq Adapter, Index 4 (96% over 32bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCAGATCGGAAGAGCA2060.11150746179786836No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGACAGAATTTAGG1930.10447058314072134TruSeq Adapter, Index 4 (97% over 37bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGAGATCGGAAGAGCACA1850.1001401962747847No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)