FastQCFastQC Report
Thu 4 Jun 2026
659_dwi-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_dwi-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543963
Total Bases82.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC72

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTAGT32541659.82318650349381TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTGGT9057616.651132521881085TruSeq Adapter, Index 13 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTAGTA70921.3037651457911659TruSeq Adapter, Index 13 (97% over 35bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG29310.5388234126218143No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTAGTATG29280.538271904522918TruSeq Adapter, Index 13 (96% over 33bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA25880.4757676533146556No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC22790.41896231912832305No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTGGTA19440.35737724808488813TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTGGG16650.30608699488751995TruSeq Adapter, Index 13 (97% over 36bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC14540.2672975919318042No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCGAGT13910.2557159218549791TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATGTAGT11530.21196294600919546TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATTTAGT11400.20957307758064425TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCGGGT10060.18493904916327028TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATTTGGT9450.1737250511523762TruSeq Adapter, Index 13 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA9430.17335737908644522No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATGTGGT9030.16600393776782613TruSeq Adapter, Index 13 (97% over 36bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAGATCGGAAGAGCAC7850.1443112858778998No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATATAGT7770.14284059761417597TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTAGTA7310.134384140097764TruSeq Adapter, Index 4 (96% over 30bp)
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC7170.13181043563624734No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTGGTATG7140.13125892753735088TruSeq Adapter, Index 13 (96% over 33bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTAGTAT6630.12188328985611155TruSeq Adapter, Index 13 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTAGG6540.12022876555942223TruSeq Adapter, Index 13 (97% over 36bp)
GATCGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTAGTA6280.11544902870231982TruSeq Adapter, Index 13 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGAAGTATATCTAGT6160.11324299630673411TruSeq Adapter, Index 13 (97% over 36bp)
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGA6130.11269148820783766No Hit
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGACGTATATCTAGT5620.10331585052659831TruSeq Adapter, Index 4 (97% over 35bp)
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGCA5520.10147749019694355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)