FastQCFastQC Report
Thu 4 Jun 2026
659_e0S-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_e0S-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233173
Total Bases35.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGT16776171.94700930210615TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTGGT217779.339417514034643TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGG74273.185188679649874TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTGGG60072.5761987880243424TruSeq Adapter, Index 6 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTG14430.6188538124053814TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTA12610.5408001784083063TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATATAGT6280.2693279239019955TruSeq Adapter, Index 6 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTATG6070.2603217353638715TruSeq Adapter, Index 6 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTGTG5680.2435959566502125TruSeq Adapter, Index 6 (97% over 34bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA4680.2007093445639075No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGT4000.17154644834522007TruSeq Adapter, Index 6 (97% over 35bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGT3280.14066808764308047TruSeq Adapter, Index 6 (97% over 35bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT3050.1308041668632303No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATATGGT2910.12480004117114761TruSeq Adapter, Index 6 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTGGTG2620.11236292366611914TruSeq Adapter, Index 6 (97% over 36bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2600.11150519142439305No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC2570.11021859306180389No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATGTAGT2380.10207013676540595TruSeq Adapter, Index 6 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)