FastQCFastQC Report
Thu 4 Jun 2026
659_eKx-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_eKx-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397977
Total Bases60 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTAGT24729562.1380130007513TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTGGT5855014.711905461873426TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTGGG102912.5858278242209978TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTAGG90262.2679702595878655TruSeq Adapter, Index 7 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTAGTA52081.3086183372405942TruSeq Adapter, Index 7 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTAGTATG21440.5387245996627945TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTGGTA12200.3065503785394633TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCGAGT9780.24574284443573371TruSeq Adapter, Index 7 (97% over 38bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG8970.22538990946713No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATATAGT8580.21559034818595044TruSeq Adapter, Index 7 (97% over 38bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA7920.19900647524856963No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC7720.1939810592069391No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCGGGT7030.17664337386331372TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATGTAGT6120.15377773087389474TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTAGTA5650.14196800317606295TruSeq Adapter, Index 7 (96% over 30bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTAGT5530.1389527535510846TruSeq Adapter, Index 7 (97% over 35bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTAGTAT5120.128650650665742TruSeq Adapter, Index 7 (97% over 36bp)
GATCGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTAGTA5030.12638921344700824TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATATGGT4800.12060998499913311TruSeq Adapter, Index 7 (97% over 38bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC4710.11834854778039935No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATCTGGTATG4590.11533329815542105TruSeq Adapter, Index 7 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA4490.11282059013460577No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATGGTGCATGTGGT4470.11231804853044272TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)