FastQCFastQC Report
Thu 4 Jun 2026
659_eeB-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_eeB-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205713
Total Bases31 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGG14063668.36514950440662TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGT2296311.162639210939512TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAGGAGATCTGGG24561.193896350741081TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGGG21971.0679927860660239TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTAGG14710.7150739136564048TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAGGAGATCTGGT7480.3636133836947592TruSeq Adapter, Index 7 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6770.3290992790927165No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCGGGG6710.3261825941967693TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGGGTG6620.3218075668528484TruSeq Adapter, Index 7 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTAGT5520.26833501042714847TruSeq Adapter, Index 7 (97% over 35bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT5190.25229324349943855No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATGTGGG5030.24451541711024583TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGGA4760.23139033507848314TruSeq Adapter, Index 7 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGGGGG4720.22944587848118497TruSeq Adapter, Index 7 (96% over 32bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4000.19444565972981775No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGG3850.1871539474899496TruSeq Adapter, Index 7 (97% over 35bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3580.17402886545818688No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATTTGGG3570.17354275130886235TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATATGGG3440.16722326736764326TruSeq Adapter, Index 7 (97% over 35bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC2980.14486201649871422No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC2840.1380564184081706No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGGG2540.12347299392843428TruSeq Adapter, Index 7 (96% over 30bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG2510.12201465148046063No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGTG2450.11909796658451338TruSeq Adapter, Index 7 (97% over 34bp)
GATCGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAGAGATCTGGGG2230.1084034552993734TruSeq Adapter, Index 7 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)