Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 197292 total bases: 29791092 Q20 bases: 25771623(86.5078%) Q30 bases: 19847103(66.6209%) Read2 before filtering: total reads: 197292 total bases: 29791092 Q20 bases: 28078564(94.2515%) Q30 bases: 21562994(72.3807%) Read1 after filtering: total reads: 11970 total bases: 718953 Q20 bases: 680757(94.6873%) Q30 bases: 616130(85.6982%) Read2 after filtering: total reads: 11970 total bases: 746641 Q20 bases: 726211(97.2637%) Q30 bases: 665584(89.1438%) Filtering result: reads passed filter: 23940 reads failed due to low quality: 15192 reads failed due to too many N: 0 reads failed due to too short: 355452 reads with adapter trimmed: 205166 bases trimmed due to adapters: 8161275 Duplication rate: 53.4061% Insert size peak (evaluated by paired-end reads): 36 JSON report: 659_egp-T1-TRNA-1_B23MHV2LT4_1.fastp.json HTML report: 659_egp-T1-TRNA-1_B23MHV2LT4_1.fastp.html fastp --in1 659_egp-T1-TRNA-1_B23MHV2LT4_1_1.fastq.gz --in2 659_egp-T1-TRNA-1_B23MHV2LT4_1_2.fastq.gz --out1 659_egp-T1-TRNA-1_B23MHV2LT4_1_1.fastp.fastq.gz --out2 659_egp-T1-TRNA-1_B23MHV2LT4_1_2.fastp.fastq.gz --json 659_egp-T1-TRNA-1_B23MHV2LT4_1.fastp.json --html 659_egp-T1-TRNA-1_B23MHV2LT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 2 seconds