FastQCFastQC Report
Thu 4 Jun 2026
659_egp-T1-TRNA-1_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_egp-T1-TRNA-1_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197292
Total Bases29.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGT8575743.46704377268212TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTGGT2971815.06295237515966TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGG2756113.96964904811143TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTGGG2431612.324878859761167TruSeq Adapter, Index 6 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGTA8810.4465462360359264TruSeq Adapter, Index 6 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGTATG5830.29550108468665737TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGTG4790.24278734059161042TruSeq Adapter, Index 6 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTGGGG3880.19666281450844433TruSeq Adapter, Index 6 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA3180.16118240982908583No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATATAGT3160.16016868398110415TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATATAGG3060.1551000547411958TruSeq Adapter, Index 6 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGTGTG3000.15205887719725078TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGGG2950.1495245625772966TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCGAGT2850.14445593333738824TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCGGGG2720.13786671532550737TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCGAGG2710.13735985240151655TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATATGGG2660.13482553778156237TruSeq Adapter, Index 6 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTGGTA2610.13229122316160816TruSeq Adapter, Index 6 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTGGTG2590.1312774973136265TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATGTGGG2370.12012651298582812TruSeq Adapter, Index 6 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCTAGGA2320.11759219836587392TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATATGGT2300.11657847251789226TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATGTAGT2260.11455102082192892TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATCGGGT2160.10948239158202057TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATGTGGT2060.10441376234211219TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGTAACATGTAGG2020.10238631064614885TruSeq Adapter, Index 6 (97% over 38bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC2000.10137258479816719No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)