Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA Read1 before filtering: total reads: 179938 total bases: 27170638 Q20 bases: 23178307(85.3065%) Q30 bases: 16970117(62.4576%) Read2 before filtering: total reads: 179938 total bases: 27170638 Q20 bases: 25479253(93.775%) Q30 bases: 19932289(73.3597%) Read1 after filtering: total reads: 23560 total bases: 1134035 Q20 bases: 1094927(96.5514%) Q30 bases: 1016293(89.6174%) Read2 after filtering: total reads: 23560 total bases: 2117979 Q20 bases: 2044170(96.5151%) Q30 bases: 1859514(87.7966%) Filtering result: reads passed filter: 47120 reads failed due to low quality: 18408 reads failed due to too many N: 0 reads failed due to too short: 294348 reads with adapter trimmed: 191846 bases trimmed due to adapters: 8947493 Duplication rate: 42.1606% Insert size peak (evaluated by paired-end reads): 36 JSON report: NTC_0001_0001_B23MHV2LT4_1.fastp.json HTML report: NTC_0001_0001_B23MHV2LT4_1.fastp.html fastp --in1 NTC_0001_0001_B23MHV2LT4_1_1.fastq.gz --in2 NTC_0001_0001_B23MHV2LT4_1_2.fastq.gz --out1 NTC_0001_0001_B23MHV2LT4_1_1.fastp.fastq.gz --out2 NTC_0001_0001_B23MHV2LT4_1_2.fastp.fastq.gz --json NTC_0001_0001_B23MHV2LT4_1.fastp.json --html NTC_0001_0001_B23MHV2LT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 2 seconds