FastQCFastQC Report
Thu 4 Jun 2026
NTC_0001_0001_B23MHV2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23MHV2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179938
Total Bases27.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGG11836865.78265847125121TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGT140027.7815692071713585TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTAGG71243.9591414820660447TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGGG26661.4816214473874334TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTAGT19761.0981560315219687TruSeq Adapter, Index 2 (97% over 36bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG8580.4768309084240127No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC7160.3979148373328591No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCGGGG6650.36957174137758564TruSeq Adapter, Index 2 (97% over 36bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA6360.3534550789716458No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGGGTG6030.3351154286476453TruSeq Adapter, Index 2 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGGGGG5100.28343095955273484TruSeq Adapter, Index 2 (96% over 33bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA4680.2600895864130978No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATTTGGG4650.25842234547455234TruSeq Adapter, Index 2 (97% over 36bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC4630.257310851515522No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATGTGGG4390.243972924007158TruSeq Adapter, Index 2 (97% over 36bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3270.1817292623014594No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATATGGG2760.1533861663461859TruSeq Adapter, Index 2 (97% over 36bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2610.1450499616534584No Hit
GATCGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGGG2550.14171547977636742TruSeq Adapter, Index 2 (96% over 31bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGGG2420.13449076904267027TruSeq Adapter, Index 13 (96% over 30bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGG2230.12393157643188209TruSeq Adapter, Index 13 (97% over 35bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAGATCGGAAGAGCAC2220.12337582945236693No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC2220.12337582945236693No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGA2110.11726261267770009No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGTG1970.10948215496448778TruSeq Adapter, Index 2 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCTGGGA1910.10614767308739677TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGATTGGATCGAGG1850.10281319121030576TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)