FastQCFastQC Report
Thu 4 Jun 2026
NTC_0001_0001_B23MHV2LT4_2_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23MHV2LT4_2_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260893
Total Bases39.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGT14293254.78567841988862TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGT4269116.36341335336709TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTAGT90943.4857201994687474TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGG48881.8735650247419438TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTGGT40031.5343454979627662TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGG28851.1058173274100878TruSeq Adapter, Index 1 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTA19550.7493493501167146TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCATCATCTATCTAGT16460.630909989919239TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTCATCATCTATCTAGT12770.489472695702836TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCATCATCTATCTGGT9640.36950013990409863TruSeq Adapter, Index 12 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTATG9140.3503351948883259TruSeq Adapter, Index 1 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTCATCATCTATCTGGT5950.22806284568769572TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCGAGTCACCTCATCATCTATCTAGT5780.22154676438233298TruSeq Adapter, Index 12 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA5480.21004779737286935No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGTA5010.19203274905804293TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGCTCATCATCTATCTAGT4490.1721012062416393TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCGAGTCACCTCATCATCTATCTGGT4390.16826821723848476TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGCTCATCATCTATCTGGT3710.1422038920170338TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTGGG3620.13875420191419469TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCATCATCTATCTGGG3500.13415461511040924TruSeq Adapter, Index 12 (97% over 35bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT3480.13338801730977834No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC3330.1276385338050465No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3110.1192059579981065No Hit
GATCGGAAGAGCACACGTCTGAACTCTGGTCACCTCATCATCTATCTAGT3100.11882265909779104Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTAGG3060.11728946349652922TruSeq Adapter, Index 12 (97% over 35bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3030.11613956679558286No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGT2930.11230657779242831TruSeq Adapter, Index 12 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)