FastQCFastQC Report
Thu 4 Jun 2026
659_8h-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_8h-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37008
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC73

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21355.769022913964548No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC11393.077712926934717No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC11323.0587980977086033No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC8612.3265239948119327No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC7462.0157803718115No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC6861.8536532641591008No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA5121.3834846519671422No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC2880.7782101167315175No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC2680.724167747514051No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC2530.6836359706009512No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC2370.6404020752269779No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC2170.5863597060095115No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2130.5755512321660182No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2000.5404236921746649No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1790.4836792044963251No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1690.4566580198875919No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1620.4377431906614786No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1570.42423259835711197No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1450.3918071768266321No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG1380.3728923476005188No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1360.36748811067877213No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC1330.3593817552961522No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG1200.324254215304799No Hit
CCGCCCCCCGGCCCCGCGTCC1190.3215520968439256No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG1170.316147859922179No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC1060.2864245568525724No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG1010.27291396454820577No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA1000.27021184608733245No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC990.26750972762645914No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG990.26750972762645914No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC980.2648076091655858No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC920.24859489840034585No Hit
CCCGCCCCCCGGCCCCGCGTCC890.2404885430177259No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT880.23778642455685256No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC860.23238218763510593No Hit
CCATCTTTCGGGTCCGCCCCCGGGGC810.2188715953307393No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG810.2188715953307393No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG740.199956766104626No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG730.1972546476437527No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA720.19455252918287938No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA690.1864461738002594No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG680.18374405533938606No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG680.18374405533938606No Hit
GGACGCGGGGCCGGGGGGCGG670.18104193687851275No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGT650.17563769995676612No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC640.17293558149589278No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG640.17293558149589278No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG630.17023346303501946No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG630.17023346303501946No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG620.16753134457414612No Hit
GCCCCCCGGCCCCGCGTCC600.1621271076523995No Hit
GGGCCCCCCGGCCCCGCGTCC600.1621271076523995No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG570.15402075226977952No Hit
CGCCCCCCGGCCCCGCGTCC540.14591439688715954No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG540.14591439688715954No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC540.14591439688715954No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC530.1432122784262862No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC510.13780804150453957No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC500.13510592304366623No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC490.1324038045827929No Hit
CTCCGCCCCCCGGCCCCGCGTCC490.1324038045827929No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGGG490.1324038045827929No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCT470.12699956766104625No Hit
ACCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC470.12699956766104625No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG460.12429744920017292No Hit
TTCCCCACCGGGCCTTCCCGT460.12429744920017292No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC460.12429744920017292No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG460.12429744920017292No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC450.12159533073929961No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC450.12159533073929961No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA440.11889321227842628No Hit
CCCCGCCCCCCGGCCCCGCGTCC430.11619109381755297No Hit
GGCCCCCCCGGCAGCAGC420.11348897535667965No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG410.11078685689580631No Hit
CTTCGCCCCCCGGCCCCGCGTCC410.11078685689580631No Hit
CTTCGCGCCCCCCGTTCCTCC410.11078685689580631No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC400.10808473843493299No Hit
CCCACCCCCGCGGGGAATCCCCC400.10808473843493299No Hit
TGCCCCGGGCGTGGGGGGGGCGGC390.10538261997405966No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC380.10268050151318635No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA380.10268050151318635No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)