FastQCFastQC Report
Thu 4 Jun 2026
659_8h-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_8h-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37008
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC11493.10473411154345No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC11173.0182663207955036No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC8582.318417639429313No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC6941.8752702118460873No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6251.688824038045828No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3700.99978383052313No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCTAGTA2990.8079334198011242TruSeq Adapter, Index 10 (96% over 30bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2850.7701037613488976No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2700.7295719844357976No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2640.7133592736705577No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2610.7052529182879378No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2520.6809338521400778No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGA2380.6431041936878512No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2180.5890618244703848No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2170.5863597060095115No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1970.532317336792045No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1840.4971897968006917No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1700.45936013834846523No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1640.44314742758322523No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG1620.4377431906614786No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1430.38640293990488545No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1380.3728923476005188No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA1360.36748811067877213No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGA1240.33506268914829224No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1150.31074362300043235No Hit
GGACGCGGGGCCGGGGGGCGGA1110.2999351491569391No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1080.2918287937743191No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1050.2837224383916991No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA1040.28102031993082577No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG1030.27831820146995245No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1020.27561608300907914No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1020.27561608300907914No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1010.27291396454820577No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1000.27021184608733245No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC860.23238218763510593No Hit
GGGGAGAGAGAGAGAGAGAGGGCGCGAAGA850.22968006917423262No Hit
GATCGGAAGAGCACACGTCTGAACTCCGTCACTAAGGCTCACATCTAGTA840.2269779507133593Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG840.2269779507133593No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC810.2188715953307393No Hit
GGACGCGGGGCCGGGGGGCGGGA810.2188715953307393No Hit
GCCCCGGGGGCGGACCCGAAAGATGGA810.2188715953307393No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC760.2053610030263727No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC740.199956766104626No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC710.19185041072200604No Hit
CCGCCCCCCGGCCCCGCGTCCA700.18914829226113272No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC690.1864461738002594No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCA680.18374405533938606No Hit
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC660.17833981841763943No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC640.17293558149589278No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC630.17023346303501946No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC620.16753134457414612No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGA610.1648292261132728No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGA580.15672287073065283No Hit
GGACGCGGGGCCGGGGGGCCCA580.15672287073065283No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC560.1513186338089062No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACTAAGGCTCACATCTAGTA560.1513186338089062Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC530.1432122784262862No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC530.1432122784262862No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC510.13780804150453957No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC510.13780804150453957No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGT500.13510592304366623No Hit
GGACGCGGGGCCGGGGGGCGGAGA490.1324038045827929No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG490.1324038045827929No Hit
GGACGCGGGGCCGGGGGGCA490.1324038045827929No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG490.1324038045827929No Hit
CCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC480.12970168612191957No Hit
AGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC480.12970168612191957No Hit
ACGGGAAGGCCCGGTGGGGAAA470.12699956766104625No Hit
GGACGCGGGGCCGGGGGGCGG450.12159533073929961No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACTAAGGCTCACATCTAGTA450.12159533073929961Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGCGCCCTCTCTCTCTCTCTCTCCCCCGCA450.12159533073929961No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCCGCA450.12159533073929961No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGCA440.11889321227842628No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC440.11889321227842628No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC440.11889321227842628No Hit
GGACGCGGGGCCGGGGGGCGGGGA430.11619109381755297No Hit
GCTGCTGCCGGGGGGGCCA430.11619109381755297No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGA420.11348897535667965No Hit
GGGGGATTCCCCGCGGGGGTGGGA410.11078685689580631No Hit
GGAGGAACGGGGGGCGCGAAGA400.10808473843493299No Hit
GGACGCGGGGCCGGGGGGCGAAGA400.10808473843493299No Hit
GCCGCCCCCCCCACGCCCGGGGCAA400.10808473843493299No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC390.10538261997405966No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTAAGGCTCACATCTGGTA380.10268050151318635TruSeq Adapter, Index 10 (96% over 30bp)
GGACGCGGGGCCGGGGGGCGGCA380.10268050151318635No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC380.10268050151318635No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)