FastQCFastQC Report
Thu 4 Jun 2026
659_R1-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_R1-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69900
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC69

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC27133.8812589413447784No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC24803.547925608011445No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC21333.051502145922747No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC14162.0257510729613735No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA7411.0600858369098713No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC7181.0271816881258942No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC6590.9427753934191702No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC6210.8884120171673819No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC5290.7567954220314735No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC5040.721030042918455No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4730.6766809728183119No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC4470.6394849785407725No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC4280.6123032904148784No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC4180.597997138769671No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTAGTA3940.5636623748211731TruSeq Adapter, Index 4 (96% over 30bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC3730.5336194563662374No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG3570.5107296137339056No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3400.4864091559370529No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG3210.4592274678111588No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT3140.4492131616595136No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2980.4263233190271816No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2910.41630901287553645No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAG2810.4020028612303291No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2730.3905579399141631No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2680.3834048640915594No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC2200.3147353361945637No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG2170.31044349070100147No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC2170.31044349070100147No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC2110.30185979971387694No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC2080.29756795422031473No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG2060.2947067238912732No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2040.2918454935622318No Hit
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2000.28612303290414876No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG1990.284692417739628No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1950.27896995708154504No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1730.2474964234620887No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1690.24177396280400573No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTAGTG1630.23319027181688123TruSeq Adapter, Index 4 (96% over 30bp)
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1580.22603719599427752No Hit
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1440.20600858369098715No Hit
GGACGCGGGGCCGGGGGGCGG1400.20028612303290413No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG1400.20028612303290413No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1370.19599427753934193No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1360.19456366237482117No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG1300.18597997138769673No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC1280.18311874105865522No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC1230.1759656652360515No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1170.16738197424892703No Hit
ACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1150.16452074391988555No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1130.16165951359084407No Hit
GGACGCGGGGCCGGGGGGCGGG1110.15879828326180256No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGT1090.15593705293276108No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1090.15593705293276108No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1040.14878397711015737No Hit
CACGCATCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1040.14878397711015737No Hit
GCCCCGGGGGCGGACCCGAAAGATGG1010.14449213161659513No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC990.14163090128755365No Hit
GGAGGAACGGGGGGCGCGAAG950.13590844062947066No Hit
CGTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC940.13447782546494993No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC910.1301859799713877No Hit
AGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC890.12732474964234622No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG870.12446351931330471No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC860.12303290414878397No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGC840.12017167381974249No Hit
ACGGGAAGGCCCGGTGGGGAA840.12017167381974249No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC830.11874105865522175No Hit
AACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC810.11587982832618027No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGC810.11587982832618027No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC800.11444921316165953No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG790.11301859799713876No Hit
CGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC780.11158798283261802No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC780.11158798283261802No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC780.11158798283261802No Hit
GGAGGAACGGGGGGCGGGAAAGATGAAAG770.11015736766809728No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA760.10872675250357654No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTGGTG760.10872675250357654TruSeq Adapter, Index 4 (96% over 30bp)
GCCGGGGGGGCTGTAACACTCGGGGGGCCC760.10872675250357654No Hit
CCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC740.10586552217453506No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC740.10586552217453506No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG730.10443490701001432No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACTGTCAGGCTTATCTAGTA730.10443490701001432Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC730.10443490701001432No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC720.10300429184549358No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG720.10300429184549358No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTAG720.10300429184549358TruSeq Adapter, Index 4 (96% over 31bp)
GGGGAGAGAGAGAGAGAGAGGGCGCGAAG720.10300429184549358No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC700.10014306151645207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)