FastQCFastQC Report
Thu 4 Jun 2026
659_R1-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_R1-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69900
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC73

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32944.71244635193133No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC27513.9356223175965668No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC24803.547925608011445No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC21383.0586552217453504No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC13821.9771101573676682No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC7941.1359084406294706No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC7321.0472103004291846No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6670.9542203147353361No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC6660.9527896995708154No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC6280.8984263233190272No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5230.748211731044349No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC5080.7267525035765379No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC4590.6566523605150215No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG4330.6194563662374821No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC4230.6051502145922747No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3650.5221745350500715No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3510.502145922746781No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG3390.4849785407725322No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3150.45064377682403434No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG2970.4248927038626609No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT2960.4234620886981402No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC2870.4105865522174535No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG2740.39198855507868385No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA2210.31616595135908443No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC2150.3075822603719599No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC2110.30185979971387694No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG2080.29756795422031473No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG2080.29756795422031473No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG2060.2947067238912732No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG2000.28612303290414876No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1990.284692417739628No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGT1990.284692417739628No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1920.27467811158798283No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1750.2503576537911302No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1750.2503576537911302No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG1700.24320457796852646No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA1590.22746781115879827No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTA1450.20743919885550785No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG1400.20028612303290413No Hit
CCGCCCCCCGGCCCCGCGTCC1390.1988555078683834No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1360.19456366237482117No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1260.18025751072961374No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1250.17882689556509299No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1200.17167381974248927No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1190.17024320457796852No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC1150.16452074391988555No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGT1140.16309012875536483No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA1100.15736766809728184No Hit
CCCGCCCCCCGGCCCCGCGTCC1080.15450643776824036No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1080.15450643776824036No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGATGCGTG1030.1473533619456366No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG1010.14449213161659513No Hit
CCATCTTTCGGGTCCGCCCCCGGGGC990.14163090128755365No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC980.1402002861230329No Hit
CTTCGCGCCCCCCGTTCCTCC970.13876967095851217No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCACG930.13304721030042918No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG930.13304721030042918No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC890.12732474964234622No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCT890.12732474964234622No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC870.12446351931330471No Hit
ACCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC860.12303290414878397No Hit
TTCCCCACCGGGCCTTCCCGT850.12160228898426323No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTT820.11731044349070101No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG810.11587982832618027No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC810.11587982832618027No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC790.11301859799713876No Hit
GCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC780.11158798283261802No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG780.11158798283261802No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG770.11015736766809728No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC770.11015736766809728No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC770.11015736766809728No Hit
CTTTCATCTTTCCCGCCCCCCGTTCCTCC770.11015736766809728No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCG760.10872675250357654No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGC750.1072961373390558No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGGG750.1072961373390558No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG720.10300429184549358No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCA720.10300429184549358No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC720.10300429184549358No Hit
GTGCTGCTGCCGGGGGGGC720.10300429184549358No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC720.10300429184549358No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG710.10157367668097284No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG710.10157367668097284No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGC700.10014306151645207No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)