FastQCFastQC Report
Thu 4 Jun 2026
659_Wm-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_Wm-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30487
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC5591.8335684062059237No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA5091.6695640764916193No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC4531.4858792272115984No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT4351.4268376685144488No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGGG3841.2595532522058581TruSeq Adapter, Index 6 (96% over 30bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3301.0824285761144092No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3281.0758684029258372No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2960.9709056319086824No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC2680.8790632072686719No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2630.8626627742972414No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2100.6888181848000787No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1830.6002558467543543No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA1640.5379342014629186No Hit
CGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1460.47889264276576904No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1420.46577229638862466No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1420.46577229638862466No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGTG1370.4493718634171942TruSeq Adapter, Index 6 (96% over 30bp)
GCCGGGGGGGCTGTAACACTCGGGGGGCC1320.4329714304457638No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG1320.4329714304457638No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGCAGC1240.40673073769147505No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1190.3903303047200446No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1160.38049004493718636No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC1110.3640896119657559No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1100.3608095253714698No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC990.3247285728343228No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC960.31488831305146453No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC960.31488831305146453No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC920.3017679666743202No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC910.29848788008003413No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG880.28864762029717583No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG860.2820874471086037No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC860.2820874471086037No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT860.2820874471086037No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGC850.2788073605143176No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC850.2788073605143176No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG810.2656870141371732No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG810.2656870141371732No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG800.26240692754288714No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC800.26240692754288714No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG770.25256666776002884No Hit
GCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC770.25256666776002884No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC750.2460064945714567No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC740.2427264079771706No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGG740.2427264079771706TruSeq Adapter, Index 6 (96% over 31bp)
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC720.23616623478859844No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC690.22632597500574017No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCCC670.21976580181716798No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC660.2164857152228819No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC650.2132056286285958No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGAGCAGC640.20992554203430971No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC620.20336536884573753No Hit
ACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC600.19680519565716537No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC600.19680519565716537No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGC590.19352510906287926No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCAC570.18696493587430707No Hit
CGAGTGTTACAGCCCCCCCGGCAGCAGC540.1771246760914488No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG530.17384458949716272No Hit
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGG500.16400432971430445TruSeq Adapter, Index 5 (96% over 33bp)
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC500.16400432971430445No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG490.16072424312001837No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG470.15416406993144618No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACGCAGATGTAAATCTGGGG470.15416406993144618Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGC460.1508839833371601No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC460.1508839833371601No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC460.1508839833371601No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG450.147603896742874No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC450.147603896742874No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGC440.14432381014858792No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACGCAGATGTAAATCTGGGG420.13776363696001573Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCAGATGTAAATCTGGGA410.13448355036572965TruSeq Adapter, Index 6 (96% over 30bp)
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC400.13120346377144357No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC400.13120346377144357No Hit
TGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC390.12792337717715746No Hit
GGCTCCAGCTCCACCTGAACGGCCAGCAGCTCCTCCAGTGCAGACTCTGG390.12792337717715746No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGC390.12792337717715746No Hit
GATCGGAAGAGCACACGTCTGAACCCAGTCACGCAGATGTAAATCTGGGG390.12792337717715746Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC380.1246432905828714No Hit
GCTGCCGGGGGGGCTGTAACACTCGG380.1246432905828714No Hit
CACGCATCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC380.1246432905828714No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGAGA370.1213632039885853No Hit
GATCGGAAGAGCACACGTCTGAACTCCGTCACGCAGATGTAAATCTGGGG370.1213632039885853Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC360.11808311739429922No Hit
GATCGGAAGAGCACAGTCTGAACTCCAGTCACGCAGATGTAAATCTGGGG360.11808311739429922No Hit
GAGTGTTACAGCCCCCCCGGCAGCAGC360.11808311739429922No Hit
CCGAGTGTTACAGCCCCCCCGGCAGC350.11480303080001313No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGATGCGTG350.11480303080001313No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG350.11480303080001313No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC340.11152294420572703No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG340.11152294420572703No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC340.11152294420572703No Hit
ATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC330.10824285761144095No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC330.10824285761144095No Hit
GGGAGAGAGAGAGAGGCAGGGTGGAGGCGGGCCCAGGCCGTAGGGCGGTG320.10496277101715486No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG320.10496277101715486No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC320.10496277101715486No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGATGCGTG320.10496277101715486No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGG310.10168268442286876No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCG310.10168268442286876No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGAGA310.10168268442286876No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGTGTCGG310.10168268442286876No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)