FastQCFastQC Report
Thu 4 Jun 2026
659_Wm-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_Wm-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30487
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC75

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26768.777511726309575No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC6272.0566142946173778No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6071.991012562731656No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5571.8270082330173516No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC4511.4793190540230263No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC3321.0889887493029815No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2930.9610653721258241No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2610.8561026011086693No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC2120.6953783579886509No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1930.6330567126972152No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1850.6068160199429266No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCG1480.4854528159543412No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1410.4624922097943386No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1410.4624922097943386No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA1370.4493718634171942No Hit
GCTGCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1330.43625151704004983No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC1280.41985108406861943No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG1170.38377013153147244No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1170.38377013153147244No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG1150.3772099583429003No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC1070.3509692655886115No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG1010.331288746022895No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC960.31488831305146453No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG950.31160822645717845No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG940.30832813986289237No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG910.29848788008003413No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG880.28864762029717583No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC880.28864762029717583No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC870.28536753370288975No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC860.2820874471086037No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC850.2788073605143176No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC820.2689671007314593No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG810.2656870141371732No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC780.2558467543543149No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC780.2558467543543149No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC770.25256666776002884No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGC770.25256666776002884No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG740.2427264079771706No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG700.22960606160002625No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC680.22304588841145406No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC670.21976580181716798No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC670.21976580181716798No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGC670.21976580181716798No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC660.2164857152228819No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC610.20008528225145145No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGT610.20008528225145145No Hit
GCTGCTCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC610.20008528225145145No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG600.19680519565716537No Hit
GCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC600.19680519565716537No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG560.183684849280021No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGG560.183684849280021No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCG540.1771246760914488No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA500.16400432971430445No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTA500.16400432971430445No Hit
GCCGGGGGGGCTGTAACACTCGGGGGTTCC480.15744415652573227No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC470.15416406993144618No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG470.15416406993144618No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC470.15416406993144618No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG460.1508839833371601No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG460.1508839833371601No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG450.147603896742874No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGC420.13776363696001573No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGATGCGTG400.13120346377144357No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC390.12792337717715746No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC390.12792337717715746No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCA390.12792337717715746No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG380.1246432905828714No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG380.1246432905828714No Hit
CCACCACCACCACCACCACCACCACCACCACCACCACCACCACAGA380.1246432905828714No Hit
CCGAGTGTTACAGCCCCCCCGGCAGC380.1246432905828714No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG370.1213632039885853No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGC370.1213632039885853No Hit
GCTGCTGCCGGGGGGGCTGTAACACTC360.11808311739429922No Hit
GGCGGTGCTGCTGTTGGATGACATAATGGCGGAGGTGGAGGTGGTGGCGG360.11808311739429922No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC350.11480303080001313No Hit
CCCCGCCCCGGGCCCTCCTCGCCGCGCCCGCTCCGTCCCGCGCCCTCCAC350.11480303080001313No Hit
GCTGCCGGGGGGGCTGTAACACTCGG350.11480303080001313No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG350.11480303080001313No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC340.11152294420572703No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG330.10824285761144095No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG330.10824285761144095No Hit
CCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACC330.10824285761144095No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG330.10824285761144095No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAT330.10824285761144095No Hit
CGAGTGTTACAGCCCCCCCGGCAGCAGC320.10496277101715486No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG310.10168268442286876No Hit
CCACCACCACCACCACCACCACCACCACCACCACCACCACAGA310.10168268442286876No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT310.10168268442286876No Hit
CCGACACCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC310.10168268442286876No Hit
CACGCATCCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC310.10168268442286876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)