FastQCFastQC Report
Thu 4 Jun 2026
659_b6o-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_b6o-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37961
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC13313.506230078238192No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC11493.0267906535655014No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC10252.7001396169753167No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC5941.5647638365691103No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3340.8798503727509812No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3320.8745818076446878No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2980.7850162008377018No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2860.7534048101999421No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2700.7112562893495956No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTAGTA2590.6822791812649825TruSeq Adapter, Index 10 (96% over 32bp)
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGA2560.6743763336055425No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC2100.5531993361607966No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT1930.5084165327573036No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1920.5057822502041569No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1920.5057822502041569No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1890.49787940254471696No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1680.44255946892863723No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1630.429388056162904No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1600.42148520850346405No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1490.3925081004188509No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1460.384605252759411No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1360.35826242722794444No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGGTA1360.35826242722794444TruSeq Adapter, Index 10 (96% over 32bp)
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1340.3529938621216512No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1320.34772529701535787No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGGTG1200.31611390637759806TruSeq Adapter, Index 10 (96% over 32bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG1100.28977108084613157No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGA1080.28450251573983826No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1060.27923395063354495No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG990.2607939727615184No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC980.25815969020837176No Hit
GGACGCGGGGCCGGGGGGCGGA970.2555254076552251No Hit
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC930.2449882774426385No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG930.2449882774426385No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC910.2397197123363452No Hit
GCCCCGGGGGCGGACCCGAAAGATGGA900.23708542978319855No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTAGTG890.23445114723005192TruSeq Adapter, Index 10 (96% over 32bp)
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA880.23181686467690527No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC860.22654829957061196No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG850.22391401701746527No Hit
CCGCCCCCCGGCCCCGCGTCCA820.21601116935802533No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC820.21601116935802533No Hit
GGAGGAACGGGGGGCGCGAAGA760.20020547403914543No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC750.19757119148599878No Hit
GGACGCGGGGCCGGGGGGCGGGA740.19493690893285215No Hit
CGCGCCCTCTCTCTCTCTCTCTCCCCCGCA710.18703406127341218No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC700.18439977872026553No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG600.15805695318879903No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCCGCA600.15805695318879903No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC590.15542267063565238No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA590.15542267063565238No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC550.14488554042306578No Hit
GGACGCGGGGCCGGGGGGCA550.14488554042306578No Hit
GGGGAGAGAGAGAGAGAGAGGGCGCGAAGA540.14225125786991913No Hit
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC540.14225125786991913No Hit
GCCCCCCCGGCAGCAGCACA530.13961697531677247No Hit
ACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC530.13961697531677247No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC530.13961697531677247No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC520.13698269276362582No Hit
GCTGCTGCCGGGGGGGCCA510.13434841021047916No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC500.1317141276573325No Hit
CCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC500.1317141276573325No Hit
GGACGCGGGGCCGGGGGGCGG490.12907984510418588No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGT470.12381127999789257No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC470.12381127999789257No Hit
GGCCCCCCCGGCAGCAGCA450.11854271489159927No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC450.11854271489159927No Hit
CCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC450.11854271489159927No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC440.11590843233845263No Hit
AACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC440.11590843233845263No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGG440.11590843233845263TruSeq Adapter, Index 10 (96% over 33bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCA430.11327414978530598Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC430.11327414978530598No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGCA420.11063986723215931No Hit
CCCCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC420.11063986723215931No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT420.11063986723215931No Hit
GGCCCCCCCGGCAGCAGCACA410.10800558467901267No Hit
GGACGCGGGGCCGGGGGGCGGGGA410.10800558467901267No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACTAGCGAGGCTATCTGGGG410.10800558467901267TruSeq Adapter, Index 10 (96% over 32bp)
GGACGCGGGGCCGGGGGGCGGAGA400.10537130212586601No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA400.10537130212586601No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACTAGCGAGGCTATCTAGTA400.10537130212586601Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGGAGAGAGAGAGAGAGAGGGCGCA400.10537130212586601No Hit
CACGCATCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC400.10537130212586601No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGCA390.10273701957271937No Hit
CGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC390.10273701957271937No Hit
GCTGCTGCCGGGGGGCCCA380.10010273701957272No Hit
GCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC380.10010273701957272No Hit
CATCCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC380.10010273701957272No Hit
GGACGCGGGGCCGGGGGGCCCA380.10010273701957272No Hit
AGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC380.10010273701957272No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACTAGCGAGGCTATCTAGTA380.10010273701957272Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC380.10010273701957272No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC380.10010273701957272No Hit
GGACGCGGGGCCGGGGGGCGAAGA380.10010273701957272No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)