FastQCFastQC Report
Thu 4 Jun 2026
659_b6o-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_b6o-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37961
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC75

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27887.344379758172861No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC13563.5720871420668576No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC11513.0320592186717947No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC10162.676431073996997No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5891.5515924238033771No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC3400.8956560680698611No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC3330.8772160901978345No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA3290.8666789599852479No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC3220.8482389821132215No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC2880.7586733753062354No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC2670.7033534416901557No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC2600.6849134638181291No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1960.5163193804167435No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1950.5136850978635968No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1870.49261083743842365No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG1680.44255946892863723No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG1630.429388056162904No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1600.42148520850346405No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT1480.3898738178657043No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1470.3872395353125576No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1320.34772529701535787No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG1300.34245673190906456No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1280.33718816680277125No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC1090.28713679829298494No Hit
CCGCCCCCCGGCCCCGCGTCC1090.28713679829298494No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG1070.28186823318669163No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1060.27923395063354495No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA1000.263428255314665No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGT920.24235399488949186No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG900.23708542978319855No Hit
CCATCTTTCGGGTCCGCCCCCGGGGC890.23445114723005192No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG840.22127973446431862No Hit
GGACGCGGGGCCGGGGGGCGG830.21864545191117196No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC830.21864545191117196No Hit
CCCGCCCCCCGGCCCCGCGTCC820.21601116935802533No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC810.21337688680487868No Hit
GCCCCCCGGCCCCGCGTCC800.21074260425173202No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG800.21074260425173202No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG780.20547403914543874No Hit
CTTCGCGCCCCCCGTTCCTCC750.19757119148599878No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG740.19493690893285215No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG730.1923026263797055No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC720.18966834382655884No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA660.17386264850767894No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC600.15805695318879903No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC600.15805695318879903No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC570.15015410552935907No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA560.14751982297621244No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGT560.14751982297621244No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG550.14488554042306578No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG550.14488554042306578No Hit
GTGCTGCTGCCGGGGGGGC540.14225125786991913No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTA540.14225125786991913No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC530.13961697531677247No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC530.13961697531677247No Hit
CGCCCCCCGGCCCCGCGTCC510.13434841021047916No Hit
GGCCCCCCCGGCAGCAGC500.1317141276573325No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC500.1317141276573325No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGGG480.12644556255103923No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG470.12381127999789257No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG470.12381127999789257No Hit
ACCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC460.12117699744474593No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC450.11854271489159927No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG450.11854271489159927No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG450.11854271489159927No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTT440.11590843233845263No Hit
GCTGCTGCCGGGGGGGCC440.11590843233845263No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC430.11327414978530598No Hit
GACTGGAGTTCAGACGTGTGCTCTCCGATC420.11063986723215931Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGGGG410.10800558467901267No Hit
CTCCGCCCCCCGGCCCCGCGTCC410.10800558467901267No Hit
CTTCGCCCCCCGGCCCCGCGTCC410.10800558467901267No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCG400.10537130212586601No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCC400.10537130212586601No Hit
GTGCTGCTGCCGGGGGGGCC400.10537130212586601No Hit
CCCCGCCCCCCGGCCCCGCGTCC400.10537130212586601No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGATGCGTG400.10537130212586601No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG400.10537130212586601No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC390.10273701957271937No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGATG390.10273701957271937No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG380.10010273701957272No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCT380.10010273701957272No Hit
CCCCGTCCTCCCCCCTCCCCGGGG380.10010273701957272No Hit
GGGCCCCCCGGCCCCGCGTCC380.10010273701957272No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)