FastQCFastQC Report
Thu 4 Jun 2026
659_buj-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_buj-T1-TRNA-1_B23MHV2LT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39483
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC71

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18864.7767393561786085No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA15093.821898032064433No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC13553.431856748474027No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC7211.8261023731732644No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC6101.5449687207152445No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC4981.2613023326494948No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3700.9371121748600664No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3620.9168502899982272No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC3530.8940556695286579No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3430.8687283134513588No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC2580.6534457867943165No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG2270.5749309829546894No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2220.5622673049160398No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2070.5242762708000912No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG2010.5090798571537117No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC1880.4761542942532229No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC1870.473621558645493No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG1840.46602335182230326No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1780.45082693817592384No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC1760.44576146696046404No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC1660.4204341108831649No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG1640.4153686396677051No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC1590.40270496162905556No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG1480.37484486994402655No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC1450.3672466631208368No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCG1380.3495175138667274No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG1380.3495175138667274No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1360.3444520426512676No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1350.34191930704353773No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG1330.33685383582807793No Hit
GCCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1260.31912468657396853No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGC1250.3165919509662386No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC1210.306461008535319No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG1210.306461008535319No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1120.2836663880657498No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1090.2760681812425601No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1080.2735354456348302No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC1070.27100271002710025No Hit
GCCGGGGGGGCTGTAACACTCGGGGGTTCC1060.26846997441937037No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC990.25074082516526097No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG930.23554441151888156No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCG880.22288073348023202No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGC870.22034799787250212No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGC860.21781526226477216No Hit
GCTGCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC860.21781526226477216No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC830.21021705544158245No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG800.20261884861839272No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC780.1975533774029329No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG770.195020641795203No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGG760.19248790618747308No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG730.18488969936428337No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG720.18235696375655347No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCA720.18235696375655347No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGG710.17982422814882354No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG700.17729149254109364No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC690.1747587569333637No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC680.1722260213256338No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG680.1722260213256338No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC670.1696932857179039No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC670.1696932857179039No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG630.15956234328698427No Hit
GCTGCCGGGGGGGCTGTAACACTCGG620.15702960767925436No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCA610.15449687207152446No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGC580.14689866524833473No Hit
CTTTAATCGTTTGTTCAACGATTAAAG560.1418331940328749No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC560.1418331940328749No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG560.1418331940328749No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGT560.1418331940328749No Hit
CCGAGTGTTACAGCCCCCCCGGCAGC550.139300458425145No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG540.1367677228174151No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGC540.1367677228174151No Hit
GCTGCCGGGGGGGCTGTAACACTCGGG530.13423498720968519No Hit
CCGCCCCCCGGCCCCGCGTCC530.13423498720968519No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC520.13170225160195528No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG510.12916951599422538No Hit
CACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCA510.12916951599422538No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAG510.12916951599422538No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCA500.12663678038649545No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG500.12663678038649545No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCGG490.12410404477876553No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC490.12410404477876553No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTA480.12157130917103563No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA470.11903857356330573No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG470.11903857356330573No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG450.11397310234784591No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC440.11144036674011601No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC440.11144036674011601No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC430.10890763113238608No Hit
TTCCCCACCGGGCCTTCCCGT430.10890763113238608No Hit
GCTGCTCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC430.10890763113238608No Hit
CACGCATCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC430.10890763113238608No Hit
TATGACAACACTGGCAGAGCCCACCTAATGGCTCCCACTCTACCTGCTCC420.10637489552465618No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGATGCGTG420.10637489552465618No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGC410.10384215991692627No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGC410.10384215991692627No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG410.10384215991692627No Hit
CTTCGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC410.10384215991692627No Hit
GCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC400.10130942430919636No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA400.10130942430919636No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAG400.10130942430919636No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)