FastQCFastQC Report
Thu 4 Jun 2026
659_buj-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_buj-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39483
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA11102.811336524580199No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT10612.6872324798014335No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC7541.9096826482283515No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC7231.8311678443887245No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC6071.5373705138920548No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG5831.476584859306537No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC4941.251171390218575No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC3730.944710381683256No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3500.8864574627054682No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC3480.8813919914900085No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCC2580.6534457867943165No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC2230.5648000405237696No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2210.5597345693083099No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2100.5318744776232809No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2020.5116125927614417No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGTG1990.5040143859382519TruSeq Adapter, Index 1 (96% over 29bp)
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1850.4685560874300332No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC1820.46095788060684345No Hit
GCTGCCGGGGGGGCTGTAACACTCGGCAGC1800.45589240939138365No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG1790.45335967378365377No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGC1760.44576146696046404No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1700.4305650533140845No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1620.41030316845224524No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1570.39763949041359575No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1480.37484486994402655No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1450.3672466631208368No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC1390.35205024947445734No Hit
CGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1360.3444520426512676No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC1330.33685383582807793No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1320.334321100220348No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1310.33178836461261807No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCCC1270.32165742218169846No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC1240.3140592153585087No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG1200.3039282729275891No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1200.3039282729275891No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCG1110.2811336524580199No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG1110.2811336524580199No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGTA1110.2811336524580199TruSeq Adapter, Index 1 (96% over 29bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC1100.27860091685029No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG1090.2760681812425601No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1080.2735354456348302No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGC1020.25833903198845076No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC980.24820808955753107No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC940.23807714712661146No Hit
CGAGTGTTACAGCCCCCCCGGCAGCAGC890.22541346908796192No Hit
GCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC860.21781526226477216No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGC860.21781526226477216No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC820.20768431983385255No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC780.1975533774029329No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG770.195020641795203No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGCAGC770.195020641795203No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG760.19248790618747308No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTGGTG760.19248790618747308TruSeq Adapter, Index 1 (96% over 29bp)
CCGAGTGTTACAGCCCCCCCGGCAGCAGCAC740.18742243497201327No Hit
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC740.18742243497201327No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC740.18742243497201327No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC710.17982422814882354No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT710.17982422814882354No Hit
TGCCGGGGGGGCTGTAACACTCGGGGGGCC680.1722260213256338No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC680.1722260213256338No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC670.1696932857179039No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC670.1696932857179039No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG650.1646278145024441No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG650.1646278145024441No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC630.15956234328698427No Hit
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA630.15956234328698427No Hit
CCGAGTGTTACAGCCCCCCCGGCAGC620.15702960767925436No Hit
TGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC620.15702960767925436No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGC600.15196413646379456No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC560.1418331940328749No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG560.1418331940328749No Hit
GGACGCGGGGCCGGGGGGCGG550.139300458425145No Hit
ACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC550.139300458425145No Hit
GCCGGGGGGGCTGTAACACTCGGGGGTTCC540.1367677228174151No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC540.1367677228174151No Hit
GCTGCCGGGGGGGCTGTAACACTCGG540.1367677228174151No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAG540.1367677228174151No Hit
CTTTAATCGTTGAACAAACGATTAAAG530.13423498720968519No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG520.13170225160195528No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGC520.13170225160195528No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG510.12916951599422538No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC510.12916951599422538No Hit
TGCCGGGGGGGCTGTAACACTCGGGGGGCCC500.12663678038649545No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAG500.12663678038649545TruSeq Adapter, Index 1 (96% over 30bp)
CCGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC490.12410404477876553No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGC490.12410404477876553No Hit
CGGGGGGGCTGTAACACTCGGGGGGCC490.12410404477876553No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC480.12157130917103563No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC480.12157130917103563No Hit
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC470.11903857356330573No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG440.11144036674011601No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGC440.11144036674011601No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGATGCGTG440.11144036674011601No Hit
GCCGGGGGGGCTGTAACACTCGGGGGG430.10890763113238608No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGC430.10890763113238608No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG430.10890763113238608No Hit
ACGGGAAGGCCCGGTGGGGAA430.10890763113238608No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGAGCAGC420.10637489552465618No Hit
GAGCAGAAATCTTGCCACTGCACACCAGCCTAGGCGACAAGAGCAAAACT410.10384215991692627No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTGGGG410.10384215991692627TruSeq Adapter, Index 1 (96% over 29bp)
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC410.10384215991692627No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC410.10384215991692627No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC400.10130942430919636No Hit
CACGCATCCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC400.10130942430919636No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCGAAG400.10130942430919636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)