FastQCFastQC Report
Thu 4 Jun 2026
659_cAa-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename659_cAa-T1-TRNA-1_B23MHV2LT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20937
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC4332.0681090891722786No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTAGTA4302.0537803887854036TruSeq Adapter, Index 23 (96% over 31bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC3481.6621292448774896No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3461.6525767779529064No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA3181.5188422410087405No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC2621.251373167120409No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC2251.0746525290156184No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT1980.9456942255337442No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1210.577924248937288No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1140.5444906147012466No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTGGTA1120.5349381477766633TruSeq Adapter, Index 23 (96% over 31bp)
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGA1090.5206094473897884No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1090.5206094473897884No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1020.487175813153747No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC960.45851841237999713No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG850.4059798442947891No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACCCAACTGTATATCTAGTA840.4012036108324975Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCTGCCGGGGGGGCTGTAACACTCGGCAGCA790.37732244352103933No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC760.3629937431341644No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG690.3295601088981229No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA650.31045517504895637No Hit
GGTTGTGGAGGTTGTGGTGGCTGTGGTGGCTGCGGTGGTGGCTGTGGTGG640.30567894158666475No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC640.30567894158666475No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC630.3009027081243731No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACCCAACTGTATATCTAGTA610.29135024119978986Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC610.29135024119978986No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC570.2722453073506233No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC550.26269284042604No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG520.24836414003916513No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC520.24836414003916513No Hit
GGACGCGGGGCCGGGGGGCGGA510.2435879065768735No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG510.2435879065768735No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACCCAACTGTATATCTAG500.23881167311458185TruSeq Adapter, Index 23 (96% over 32bp)
GATCGGAAGAGCACAGTCTGAACTCCAGTCACCCAACTGTATATCTAGTA500.23881167311458185TruSeq Adapter, Index 23 (95% over 21bp)
GATCGGAAGAGCACCGTCTGAACTCCAGTCACCCAACTGTATATCTAGTA490.2340354396522902TruSeq Adapter, Index 2 (95% over 21bp)
CCACCACCACACCACCACCACCACCACCACCACCACCACCACCACCACCA480.22925920618999857No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC480.22925920618999857No Hit
GCCCCGGGGGCGGACCCGAAAGATGGA480.22925920618999857No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGA450.21493050580312367No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC440.21015427234083203No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACCCAACTGTATATCTAG400.19104933849166547Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC400.19104933849166547No Hit
CCGCCCCCCGGCCCCGCGTCCA380.1814968715670822No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC360.1719444046424989No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC350.1671681711802073No Hit
CGCGCCCTCTCTCTCTCTCTCTCCCCCGCA350.1671681711802073No Hit
GGGGAGAGAGAGAGAGAGAGGGCGCGAAGA350.1671681711802073No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC350.1671681711802073No Hit
GATCGGAAGAGCACACTCTGAACTCCAGTCACCCAACTGTATATCTAGTA350.1671681711802073No Hit
CCCCGGGGAGGGGGGAGGACGGGGA340.16239193771791566No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG330.15761570425562402No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC330.15761570425562402No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCCGCA330.15761570425562402No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGA330.15761570425562402No Hit
GATCGGAAGAGCACACGTCTGAACTCCGTCACCCAACTGTATATCTAGTA310.14806323733104074Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGTACTCGCCGCAGAGGCCAGCTGCCTCGACGAACTCCTCCTGTGTCAT310.14806323733104074No Hit
GGACGCGGGGCCGGGGGGCGGAGA310.14806323733104074No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC310.14806323733104074No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGA300.1432870038687491No Hit
GATCGGAAGAGCACACGTCTGAATCCAGTCACCCAACTGTATATCTAGTA290.13851077040645746Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ACGTGCCCTCCCTGTCGGCACCCCAGCGCCCGCCCGGCACCACCCAGAAC290.13851077040645746No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC290.13851077040645746No Hit
GATCGGAAGAGCACACGTCCTGAACTCCAGTCACCCAACTGTATATCTAG280.13373453694416582No Hit
GATGTTTTTTAGGTAAGGATAGATACATTAGGCTTGCTTTGTGAAAGTAA280.13373453694416582No Hit
CTAAAGTAATAAATAAATGCTTGAAATATACCCAGTAGAAACTTATTTTT280.13373453694416582No Hit
GATCGGAAGAGCAACGTCTGAACTCCAGTCACCCAACTGTATATCTAGTA280.13373453694416582TruSeq Adapter, Index 23 (95% over 23bp)
GATCGGAAGAGCACACGCTGAACTCCAGTCACCCAACTGTATATCTAGTA280.13373453694416582No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC280.13373453694416582No Hit
ACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC270.1289583034818742No Hit
GATCGGAAGAGCACACGTCGAACTCCAGTCACCCAACTGTATATCTAGTA270.1289583034818742No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACCCAACTGTATATCTGGTA270.1289583034818742Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC260.12418207001958256No Hit
GATCGGAAGAGCACACGTCTGAACCCAGTCACCCAACTGTATATCTAGTA260.12418207001958256Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGGGGAGAGAGAGAGAGAGAGGGCGCA260.12418207001958256No Hit
GCCCCCCCGGCAGCAGCACA250.11940583655729092No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCA250.11940583655729092Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG250.11940583655729092No Hit
GGACGCGGGGCCGGGGGGCA250.11940583655729092No Hit
GACGCGGGGCCGGGGGGCGAAGA250.11940583655729092No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG250.11940583655729092No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTC250.11940583655729092Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGGC250.11940583655729092No Hit
GATCGGAAGAGCACACGTTGAACTCCAGTCACCCAACTGTATATCTAGTA240.11462960309499928No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC240.11462960309499928No Hit
GATCGGAAGAGCACACGTCTAACTCCAGTCACCCAACTGTATATCTAGTA240.11462960309499928Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTCTTTCCAGGCTCAGGGGGCAAGTCACGTGGCAGGGGGAGCCCTGCTGG240.11462960309499928No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGCCCA230.10985336963270766No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC230.10985336963270766No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC230.10985336963270766No Hit
GGACGCGGGGCCGGGGGGCGGGA230.10985336963270766No Hit
CTCGCGGGGGATTCCCCGCGAGG230.10985336963270766No Hit
GGCCCCCCCGGCAGCAGCACA230.10985336963270766No Hit
GCTGCAGCGCCTCCGTGTCCCCTTCCTGGTGGCTGTTCCCCTCCTGGGGC220.10507713617041602No Hit
GGAGGAACGGGGGGCGCGAAGA220.10507713617041602No Hit
GGACGCGGGGCCGGGGGGCGG220.10507713617041602No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC220.10507713617041602No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC220.10507713617041602No Hit
GATCGGAAGAGCACACCGTCTGAACTCCAGTCACCCAACTGTATATCTAG220.10507713617041602TruSeq Adapter, Index 23 (95% over 22bp)
CGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC210.10030090270812438No Hit
TGGAATGGGGCCGCCCGGCTCCCCATGGCAGTGGGTGACGCTGCTGCTGG210.10030090270812438No Hit
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT210.10030090270812438No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)